Guda Chittibabu, Guda Purnima, Fahy Eoin, Subramaniam Shankar
San Diego Supercomputer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W372-4. doi: 10.1093/nar/gkh374.
MITOPRED web server enables prediction of nucleus-encoded mitochondrial proteins in all eukaryotic species. Predictions are made using a new algorithm based primarily on Pfam domain occurrence patterns in mitochondrial and non-mitochondrial locations. Pre-calculated predictions are instantly accessible for proteomes of Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila, Homo sapiens, Mus musculus and Arabidopsis species as well as all the eukaryotic sequences in the Swiss-Prot and TrEMBL databases. Queries, at different confidence levels, can be made through four distinct options: (i) entering Swiss-Prot/TrEMBL accession numbers; (ii) uploading a local file with such accession numbers; (iii) entering protein sequences; (iv) uploading a local file containing protein sequences in FASTA format. Automated updates are scheduled for the pre-calculated prediction database so as to provide access to the most current data. The server, its documentation and the data are available from http://mitopred.sdsc.edu.
MITOPRED网络服务器能够预测所有真核生物物种中由细胞核编码的线粒体蛋白质。预测是使用一种新算法进行的，该算法主要基于线粒体和非线粒体位置的Pfam结构域出现模式。对于酿酒酵母、秀丽隐杆线虫、果蝇、智人、小家鼠和拟南芥物种的蛋白质组以及瑞士蛋白质数据库（Swiss-Prot）和欧洲生物信息学研究所蛋白质数据库（TrEMBL）中的所有真核序列，预先计算的预测结果可即时获取。可以通过四种不同选项进行不同置信水平的查询：（i）输入瑞士蛋白质数据库/欧洲生物信息学研究所蛋白质数据库的登录号；（ii）上传包含此类登录号的本地文件；（iii）输入蛋白质序列；（iv）上传包含FASTA格式蛋白质序列的本地文件。预先计算的预测数据库计划进行自动更新，以便提供对最新数据的访问。该服务器、其文档和数据可从http://mitopred.sdsc.edu获取。
