Raza Yasir, Khan Adnan, Khan Asif Iqbal, Khan Saeed, Akhter Shakeel, Mubarak Muhammad, Ahmed Ayaz, Kazmi Shahana Urooj
Stem Cell Research Laboratory, Sindh Institute of Urology and Transplantation, Deewan Farooq Medical Complex, Chand Bibi Road, Karachi, Pakistan.
Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Karachi, Pakistan.
Pathol Oncol Res. 2017 Oct;23(4):873-880. doi: 10.1007/s12253-017-0191-9. Epub 2017 Jan 21.
Helicobacter pylori is one of the major risk factors involved in the development ofgastritis and gastric cancer (GC). H. pylori infection leads to increased production of pro-inflammatory cytokines by the host. Carriage of specific polymorphisms in cytokine genes may be associated with host susceptibility to the development of GC. We investigated the role of host genetic factors including polymorphisms of IL-1B and IL-1RN in correlation with gastritis and GC in H. pylori infected Pakistani population. A total of 230 gastritis cases and 100 GC cases were genotyped for IL 1B-511 and IL-1RN penta-allelic variable number of tandem repeats (VNTRs). A combination of IL-1B-511T and IL-1RN2 alleles (OR 19.064; 95% CI 2.319-156.7; p = 0.001) in H. pylori infected individuals had markedly increased risk of GC development. In Pakistani population, an increased risk of GC development is associated with the carriage of IL-1B-511T and IL-1RN2 alleles. Synergistic effect of H. pylori infection and IL-1B-511T/IL-1RN2 genotypes was also observed in association with significantly higher risk of developing GC. Further prospective and large scale studies are needed to establish the clinical impact of these findings.
幽门螺杆菌是胃炎和胃癌（GC）发生的主要危险因素之一。幽门螺杆菌感染会导致宿主促炎细胞因子的产生增加。细胞因子基因中特定多态性的携带可能与宿主对GC发生的易感性有关。我们研究了包括白细胞介素-1β（IL-1B）和白细胞介素-1受体拮抗剂（IL-1RN）多态性在内的宿主遗传因素与幽门螺杆菌感染的巴基斯坦人群中胃炎和GC的相关性。对总共230例胃炎病例和100例GC病例进行了IL-1B -511和IL-1RN五等位基因串联重复序列可变数目（VNTRs）的基因分型。在幽门螺杆菌感染个体中，IL-1B -511T和IL-1RN2等位基因的组合（比值比19.064；95%置信区间2.319 - 156.7；p = 0.001）有显著增加的GC发生风险。在巴基斯坦人群中，GC发生风险增加与IL-1B -511T和IL-1RN2等位基因的携带有关。还观察到幽门螺杆菌感染与IL-1B -511T/IL-1RN2基因型的协同作用与发生GC的显著更高风险相关。需要进一步的前瞻性和大规模研究来确定这些发现的临床影响。
