van der Merwe J P, Kruger T F, Swart Y, Lombard C J
Tygerberg Hospital, University of Stellenbosch, South Africa.
Fertil Steril. 1992 Sep;58(3):581-6. doi: 10.1016/s0015-0282(16)55267-4.
To determine the effect of oocyte maturity on pregnancy rate (PR) in patients undergoing gamete intrafallopian transfer (GIFT) procedures for infertility and in whom male partners had either normozoospermia or teratozoospermia.
A prospective study using a combination of clomiphene citrate and human menopausal gonadotropin for ovulation induction was carried out. Five hundred thirty-seven GIFT treatment cycles were analyzed, and 423 cycles yielded four or more oocytes. Patients were divided into two groups according to the sperm morphology: those with less than 14% normal forms and those with greater than 14% normal morphology (as judged by strict criteria). These two groups were divided into a further five subcategories depending on the number of metaphase II oocytes transferred.
In the group less than 14% normal forms (category I; 4 metaphase I oocytes replaced), three pregnancies resulted from 33 cycles (9.09%), and in category V (4 metaphase II oocytes transferred) the PR was 15% (14/95) per cycle. In the group with greater than 14% normal forms, in category I, the PR was 16% (3/19) per cycle, and in category V, the PR was 33% (20/61) per cycle. The effect of morphology (less than 14% normal forms versus greater than 14% normal forms) on pregnancy outcome is significant (P = 0.0161). The overall effect of increasing the number of metaphase II oocytes on the pregnancy outcome was not significant (P = 0.1098). However, in the group with greater than 14% normal forms, there was a significant improvement in PR (P = 0.04) in category V.
During this prospective study, it was shown that sperm morphology plays a significant role in the pregnancy outcome in a GIFT program.
确定卵母细胞成熟度对接受配子输卵管内移植（GIFT）治疗不孕症患者妊娠率（PR）的影响，这些患者的男性伴侣患有正常精子症或畸形精子症。
进行一项前瞻性研究，联合使用枸橼酸氯米芬和人绝经期促性腺激素诱导排卵。分析了537个GIFT治疗周期，其中423个周期产生了4个或更多卵母细胞。根据精子形态将患者分为两组：正常形态低于14%的患者和正常形态高于14%的患者（根据严格标准判断）。根据移植的中期II卵母细胞数量，这两组又进一步分为五个亚组。
在正常形态低于14%的组（I类；移植4个中期I卵母细胞）中，33个周期中有3例妊娠（9.09%），在V类（移植4个中期II卵母细胞）中，每个周期的妊娠率为15%（14/95）。在正常形态高于14%的组中，I类每个周期的妊娠率为16%（3/19），V类每个周期的妊娠率为33%（20/61）。形态（正常形态低于14%与高于14%）对妊娠结局的影响具有显著性（P = 0.0161）。增加中期II卵母细胞数量对妊娠结局的总体影响不显著（P = 0.1098）。然而，在正常形态高于14%的组中，V类的妊娠率有显著提高（P = 0.04）。
在这项前瞻性研究中，表明精子形态在GIFT程序的妊娠结局中起着重要作用。
