Coetzee K, Windt M L
Obstetrics and Gynecology Department, Tygerberg Hospital, South Africa.
J Assist Reprod Genet. 1996 Nov;13(10):768-71. doi: 10.1007/BF02066495.
In this study we investigated whether metaphase I oocytes collected in an intracytoplasmic sperm injection program could successfully be matured and fertilized by injecting aged (> 20-hr) spermatozoa.
Metaphase I oocytes aspirated were preincubated for 20 hr to allow the oocytes to reach meiotic maturity. Only metaphase II oocytes were injected. The original sperm sample processed on the day of aspiration was used in the microinjection process.
One hundred eighty-three oocytes were collected, of which 42 (23%) were metaphase I oocytes. These were incubated for 20 hr and microinjected with the original sperm sample. Thirty-one (74%) of the metaphase I oocytes reached meiotic maturity (extruded polar body): 67.7% showed two pronuclei 18 hr after injection and 61.3% embryo development 40 hr postinjection. No difference in fertilization and embryo development rate was found in metaphase II oocytes injected 6 hr postaspiration versus 20 hr postaspiration. An ongoing pregnancy was also achieved using only embryos obtained from matured metaphase I oocytes.
Metaphase I oocytes can be successfully matured in vitro and injected using aged (> 20-hr) sperm samples. Matured metaphase I oocytes, if successfully injected, produce embryos able to induce pregnancy.
在本研究中，我们调查了在卵胞浆内单精子注射程序中收集的中期I卵母细胞，通过注射老化（>20小时）精子是否能够成功成熟并受精。
吸出的中期I卵母细胞预孵育20小时，以使卵母细胞达到减数分裂成熟。仅注射中期II卵母细胞。在抽吸当天处理的原始精子样本用于显微注射过程。
共收集了183个卵母细胞，其中42个（23%）为中期I卵母细胞。将这些卵母细胞孵育20小时，并用原始精子样本进行显微注射。31个（74%）中期I卵母细胞达到减数分裂成熟（排出极体）：67.7%在注射后18小时显示出两个原核，61.3%在注射后40小时胚胎发育。抽吸后6小时注射的中期II卵母细胞与抽吸后20小时注射的中期II卵母细胞在受精率和胚胎发育率方面未发现差异。仅使用从中期I成熟卵母细胞获得的胚胎也实现了持续妊娠。
中期I卵母细胞可在体外成功成熟，并使用老化（>20小时）的精子样本进行注射。如果成功注射，中期I成熟卵母细胞可产生能够诱导妊娠的胚胎。
