Aleksandrova E A, Onishchenko G E
Department of Cell Biology and Histology, Biological Faculty, Moscow State University.
Tsitologiia. 2003;45(8):796-803.
We have studied the influence of translation of inhibitor cycloheximide (CHI) on the Chinese hamster fibroblast line FAF28. This treatment inhibited the cell cycle and induced apoptosis. A short-term treatment and the following removal of CHI reduced the number of apoptotic cells up to the normal level. Under changed conditions (change in CO2 proportion and pH of cultural medium), the ability of CHI to induce apoptosis increases. The study of cell ultrastructure demonstrates three groups of apoptotic cells differing from each other in the state of cytoplasmic organelles: 1) cells with many well-identified organelles; 2) cells with a scanty number of cytoplasmic organelles; 3) cells lacking any recognizable organelles in the cytoplasm. Presumable causes of this morphological variety of apoptotic cells are discussed.
我们研究了翻译抑制剂环己酰亚胺（CHI）对中国仓鼠成纤维细胞系FAF28的影响。这种处理抑制了细胞周期并诱导了细胞凋亡。短期处理并随后去除CHI可将凋亡细胞数量减少至正常水平。在改变的条件下（培养基中二氧化碳比例和pH值的变化），CHI诱导细胞凋亡的能力增强。细胞超微结构研究表明，有三组凋亡细胞，它们在细胞质细胞器状态上彼此不同：1）具有许多可明确识别的细胞器的细胞；2）细胞质细胞器数量稀少的细胞；3）细胞质中缺乏任何可识别细胞器的细胞。文中讨论了凋亡细胞这种形态多样性的可能原因。
