Kim Dojung, Filtz Michael R, Proteau Philip J
College of Pharmacy, Oregon State University, Corvallis, Oregon 97331-3507, USA.
J Nat Prod. 2004 Jun;67(6):1067-9. doi: 10.1021/np049892x.
The biosynthesis of diadinoxanthin and beta-carotene in Euglena gracilis was examined using [1-13C]-D-glucose and [5,5-2H2]-1-deoxy-D-xylulose. In contrast to previous studies on isoprenoid biosynthesis in E. gracilis, the results demonstrate a role for the methylerythritol phosphate (MEP) pathway, along with the mevalonate pathway, in carotenoid biosynthesis. Interestingly, the MEP pathway is not involved in the biosynthesis of phytol, a result not previously observed for other chloroplast-containing organisms.
利用[1-¹³C]-D-葡萄糖和[5,5-²H₂]-1-脱氧-D-木酮糖研究了纤细裸藻中二异丁烯基二氢叶黄素和β-胡萝卜素的生物合成。与之前关于纤细裸藻中类异戊二烯生物合成的研究不同，结果表明甲基赤藓糖醇磷酸（MEP）途径以及甲羟戊酸途径在类胡萝卜素生物合成中发挥作用。有趣的是，MEP途径不参与叶绿醇的生物合成，这一结果在其他含叶绿体的生物体中尚未观察到。
