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在三角褐指藻和卵形菱形藻中，二氧化碳作为通过非甲羟戊酸途径的甲基赤藓糖醇4-磷酸途径进行类异戊二烯生物合成的主要碳源。

CO2 as main carbon source for isoprenoid biosynthesis via the mevalonate-independent methylerythritol 4-phosphate route in the marine diatoms Phaeodactylum tricornutum and Nitzschia ovalis.

作者信息

Cvejić J H, Rohmer M

机构信息

Université Louis Pasteur/CNRS, Institut Le Bel, Strasbourg, France.

出版信息

Phytochemistry. 2000 Jan;53(1):21-8. doi: 10.1016/s0031-9422(99)00465-3.

DOI:10.1016/s0031-9422(99)00465-3
PMID:10656403
Abstract

Isoprenoid biosynthesis was investigated in the two diatoms Phaeodactylum tricornutum and Nitzschia ovalis by labeling experiments performed in mixotrophic growth conditions with sodium [1-(13)C]acetate, 13CO2, [1-(13)C]glucose, sodium [3-(13)C]pyruvate and 1-deoxy-D-[5,5-(2)H2]xylulose. A clear dichotomy was found. Acetate was the preferred carbon source for the formation of the sterols in the cytoplasm via the mevalonate pathway. Carbon dioxide was the main source for phytol biosynthesis in the chloroplasts via the mevalonate-independent methylerythritol 4-phosphate pathway. The two diatoms showed the same compartmentation for isoprenoid biosynthesis as that previously found in higher plants, the red alga Porphyridium cruentum and the Chrysophyte Ochromonas danica.

摘要

通过在混合营养生长条件下用[1-(13)C]醋酸钠、13CO2、[1-(13)C]葡萄糖、[3-(13)C]丙酮酸钠和1-脱氧-D-[5,5-(2)H2]木酮糖进行标记实验，研究了两种硅藻三角褐指藻和卵形菱形藻中的类异戊二烯生物合成。发现了明显的二分法。醋酸盐是通过甲羟戊酸途径在细胞质中形成甾醇的首选碳源。二氧化碳是通过不依赖甲羟戊酸的甲基赤藓糖醇4-磷酸途径在叶绿体中合成叶绿醇的主要来源。这两种硅藻在类异戊二烯生物合成中的区室化与先前在高等植物、红藻紫球藻和金藻丹麦赭球藻中发现的相同。

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