Inwongwan Sahutchai, Kruger Nicholas J, Ratcliffe R George, O'Neill Ellis C
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
Metabolites. 2019 Jun 14;9(6):115. doi: 10.3390/metabo9060115.
Euglenids are a group of algae of great interest for biotechnology, with a large and complex metabolic capability. To study the metabolic network, it is necessary to know where the component enzymes are in the cell, but despite a long history of research into , the subcellular locations of many major pathways are only poorly defined. is phylogenetically distant from other commonly studied algae, they have secondary plastids bounded by three membranes, and they can survive after destruction of their plastids. These unusual features make it difficult to assume that the subcellular organization of the metabolic network will be equivalent to that of other photosynthetic organisms. We analysed bioinformatic, biochemical, and proteomic information from a variety of sources to assess the subcellular location of the enzymes of the central metabolic pathways, and we use these assignments to propose a model of the metabolic network of . Other than photosynthesis, all major pathways present in the chloroplast are also present elsewhere in the cell. Our model demonstrates how can synthesise all the metabolites required for growth from simple carbon inputs, and can survive in the absence of chloroplasts.
眼虫藻是一类对生物技术极具吸引力的藻类，具有庞大而复杂的代谢能力。为了研究代谢网络，有必要了解组成酶在细胞中的位置，但尽管对其进行了长期研究，许多主要途径的亚细胞定位仍定义不清。眼虫藻在系统发育上与其他常用作研究对象的藻类相距甚远，它们具有由三层膜包围的次生质体，并且在质体被破坏后仍能存活。这些不寻常的特征使得难以假定代谢网络的亚细胞组织与其他光合生物的相同。我们分析了来自各种来源的生物信息学、生物化学和蛋白质组学信息，以评估中心代谢途径中酶的亚细胞定位，并利用这些定位结果提出了眼虫藻代谢网络的模型。除了光合作用外，叶绿体中存在的所有主要途径在细胞的其他部位也有。我们的模型展示了眼虫藻如何从简单的碳输入合成生长所需的所有代谢物，以及如何在没有叶绿体的情况下存活。
