Yamaguchi Tetsuya, Yamazaki Tsutomu, Inoue Miyuki, Ogawa Motohiko, Shiga Sadashi, Nakagawa Yuichi, Kishimoto Toshio, Ouchi Kazunobu, Ohzeki Takehiko
Department of Pediatrics, Hamamatsu University School of Medicine, Shizuoka 431-3192, Japan.
Jpn J Infect Dis. 2004 Jun;57(3):103-6.
The purpose of the present study was to develop an optimal method for culturing Simkania negevensis. Centrifugation was effective for the propagation of S. negevensis, but sonication was not effective. The addition of cycloheximide to the culture medium significantly decreased the number of inclusions. Pretreatment of host monolayers with diethylaminoethyl-dextran or polyethylene glycol was detrimental. The most optimal conditions were centrifugation of the inocula onto untreated Vero cells, and culture in RPMI 1640 medium containing 10% fetal calf serum without cycloheximide or antimicrobial agents.
本研究的目的是开发一种培养内格夫希曼体的优化方法。离心法对内格夫希曼体的增殖有效，但超声处理无效。在培养基中添加放线菌酮可显著减少包涵体数量。用二乙氨基乙基葡聚糖或聚乙二醇对宿主单层细胞进行预处理是有害的。最优化的条件是将接种物离心接种到未处理的非洲绿猴肾细胞上，并在含有10%胎牛血清、不含放线菌酮或抗菌剂的RPMI 1640培养基中培养。
