Artigiani Stefania, Conrotto Paolo, Fazzari Pietro, Gilestro Giorgio F, Barberis Davide, Giordano Silvia, Comoglio Paolo M, Tamagnone Luca
Institute for Cancer Research and Treatment (IRCC), University of Torino--Medical School, 10060 Candiolo (TO), Italy.
EMBO Rep. 2004 Jul;5(7):710-4. doi: 10.1038/sj.embor.7400189. Epub 2004 Jun 25.
Semaphorins are a large family of molecular cues implicated in neural development and in a variety of functions outside the nervous system. Semaphorin 5A (Sema5A) is a transmembrane semaphorin, containing seven thrombospondin type-1 repeats, which was recently found to control axon guidance. Here we show that plexin-B3 is a high-affinity receptor specific for Sema5A. We further demonstrate that plexin-B3 activation by Sema5A mediates functional responses in plexin-B3-expressing cells (either fibroblasts, epithelial and primary endothelial cells). In addition, Sema5A can trigger the intracellular signalling of the hepatocyte growth factor/scatter factor receptor, Met, associated in a complex with plexin-B3. We thus conclude that Sema5A is able to elicit multiple functional responses through its receptor plexin-B3.
信号素是一大类分子信号，与神经发育以及神经系统外的多种功能有关。信号素5A（Sema5A）是一种跨膜信号素，包含7个血小板反应蛋白-1重复序列，最近发现它可控制轴突导向。在此我们表明，丛状蛋白-B3是Sema5A的高亲和力特异性受体。我们进一步证明，Sema5A激活丛状蛋白-B3可介导在表达丛状蛋白-B3的细胞（成纤维细胞、上皮细胞和原代内皮细胞）中的功能反应。此外，Sema5A可触发与丛状蛋白-B3形成复合物的肝细胞生长因子/分散因子受体Met的细胞内信号传导。因此，我们得出结论，Sema5A能够通过其受体丛状蛋白-B3引发多种功能反应。
