信号素5A及其受体丛状蛋白B3的表达促进胃癌的侵袭和转移。
Expression of semaphorin 5A and its receptor plexin B3 contributes to invasion and metastasis of gastric carcinoma.
作者信息
Pan Guo-Qing, Ren Hong-Zheng, Zhang Shu-Fang, Wang Xi-Mei, Wen Ji-Fang
机构信息
Department of Pathology, Xiangya Medical College, Central South University, Changsha, Hunan Province, China.
出版信息
World J Gastroenterol. 2009 Jun 14;15(22):2800-4. doi: 10.3748/wjg.15.2800.
Abstract
AIM
To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.
METHODS
Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting.
RESULTS
The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3.
CONCLUSION
Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.
摘要
目的
研究信号素5A及其受体丛状蛋白B3在胃癌中的蛋白和mRNA表达，并探讨其在胃癌侵袭和转移中的作用。
方法
采用逆转录-聚合酶链反应（RT-PCR）、实时RT-PCR和蛋白质印迹法检测48例原发性胃癌样本及其相应的非肿瘤性黏膜组织以及配对的区域淋巴结转移灶中信号素5A及其受体丛状蛋白B3的表达。
结果
信号素5A及其受体丛状蛋白B3的蛋白和mRNA表达在非肿瘤性黏膜、原发性胃癌及淋巴结转移灶中逐渐升高（P<0.05）。此外，信号素5A的表达与丛状蛋白B3的表达密切相关。
结论
信号素5A及其受体丛状蛋白B3在胃癌的侵袭和转移中起重要作用。
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