Paiva Ricardo Losekann, Sant'Ana Filho Manoel, Bohrer Paula Luce, Lauxen Isabel da Silva, Rados Pantelis Varvaki
Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Anal Quant Cytol Histol. 2004 Jun;26(3):175-80.
To evaluate cell proliferative activity by counting and measuring argyrophilic nucleolar organizer regions (AgNORs) per nucleus in cell smears from mucosa clinically exposed to smoking and alcohol.
Group 1 (control) consisted 17 patients, group 2 (smoking) of 25 and group 3 (smoking and alcohol) of 18. Cell smears collected from the mucosa of the lower lip, border of the tongue and floor of the mouth underwent AgNOR staining. Mean number and mean area of AgNORs per nucleus were calculated for the first 50 cells in each smear. ANOVA and the Tukey test were used for statistical analyses at a 5% significance level.
Statistical analyses revealed a greater mean number and larger mean area of AgNORs per nucleus in groups 2 (smoking) and 3 (smoking and alcohol). Samples from the border of the tongue had the lowest mean values for number and area of AgNORs per nucleus in comparison with samples from the lower lip and floor of the mouth in the 3 groups.
Anatomic sites exposed to smoking or to smoking and alcohol had increased cellular proliferative activity.
通过对临床暴露于吸烟和饮酒环境的黏膜细胞涂片进行计数和测量每个细胞核中的嗜银核仁组织区（AgNORs），评估细胞增殖活性。
第1组（对照组）由17名患者组成，第2组（吸烟组）有25名患者，第3组（吸烟和饮酒组）有18名患者。对从下唇黏膜、舌缘和口腔底部采集的细胞涂片进行AgNOR染色。计算每个涂片前50个细胞中每个细胞核的AgNORs平均数和平均面积。采用方差分析和Tukey检验进行统计学分析，显著性水平为5%。
统计学分析显示，第2组（吸烟组）和第3组（吸烟和饮酒组）每个细胞核的AgNORs平均数更高，平均面积更大。在这3组中，与下唇和口腔底部的样本相比，舌缘样本的每个细胞核的AgNORs数量和面积平均值最低。
暴露于吸烟或吸烟与饮酒环境的解剖部位细胞增殖活性增加。
