Gedoz Luhana, Lauxen Isabel da Silva, Sant'Ana Manoel Filho, Rados Pantelis Varvaki
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande of Sul, Porto Alegre, Brazil.
Anal Quant Cytol Histol. 2007 Aug;29(4):231-8.
To evaluate cell proliferation in clinically healthy oral mucosa exposed to smoking and alcohol carcinogens over a period of 24 months using the AgNOR staining technique.
Sixty patients were initially evaluated: 17 were control individuals, 25 were smokers and 18 were smokers and alcohol drinkers. Fifty-two of these patients were reevaluated. Specimens for cytology were obtained from swabs of lower lip mucosa, border of the tongue and floor of the mouth and underwent AgNOR staining for evaluation of mean number and mean area of AgNOR dots per nucleus and percentage of nuclei with > 3 and > 5 AgNOR dots. Student t and Kruskal-Wallis tests were used to compare values obtained.
A statistically significant increase was found in mean number of AgNOR dots per nucleus in 2 groups. One group showed a tendency toward increase of these values. The results of the longitudinal evaluation (Kruskal-Wallis test) revealed a statistically significant difference in number and area of AgNOR dots in the cells of the lower lip.
The increase of the variables suggests that the longitudinal evaluation of changes in cell proliferation in individuals exposed to smoking and alcohol carcinogens may be a useful monitoring tool.
运用核仁组成区嗜银蛋白（AgNOR）染色技术，评估在24个月期间暴露于吸烟和酒精致癌物的临床健康口腔黏膜中的细胞增殖情况。
最初评估了60名患者：17名是对照个体，25名是吸烟者，18名是吸烟且饮酒者。其中52名患者接受了重新评估。细胞学标本取自下唇黏膜、舌缘和口底的拭子，进行AgNOR染色以评估每个细胞核中AgNOR点的平均数和平均面积，以及具有>3个和>5个AgNOR点的细胞核百分比。使用学生t检验和克鲁斯卡尔-沃利斯检验来比较所得值。
在2组中发现每个细胞核中AgNOR点的平均数有统计学显著增加。一组显示出这些值有增加的趋势。纵向评估（克鲁斯卡尔-沃利斯检验）的结果显示下唇细胞中AgNOR点的数量和面积存在统计学显著差异。
变量的增加表明，对暴露于吸烟和酒精致癌物的个体的细胞增殖变化进行纵向评估可能是一种有用的监测工具。
