Cunningham J M, Hodgson H J
Department of Medicine, Royal Postgraduate Medical School, London, UK.
Int J Artif Organs. 1992 Mar;15(3):162-7.
Contact activation of plasma clotting may limit the use of some microcarrier types for hepatocyte attachment in a liver assist device. Activation by seven microcarrier types was studied in plasma containing 2-500 units heparin/mL. Clotting was activated by dextran (Cytodex 1 and 2) and collagen-coated (Cytodex 3) microcarriers at 2-25 units/mL (Cytodex 1) and 2-100 units/mL (Cytodex 2 and 3). There was no activation by polystyrene, gelatin, glass or fibronectin-coated polystyrene microcarriers. Compared with culture medium, incubation of HepG2 cells in plasma did not affect cell viability but increased cell number (56.4 versus 65.1 x 10(4) cells; P less than 0.05) and incorporation of [3H]-amino acids into protein (204913 versus 279624 dpm; P less than 0.05). Polystyrene-attached cells demonstrated time-linear protein synthesis, glucose and 7-ethoxycoumarin metabolism. We conclude that polystyrene-attached hepatocytes maintain viability and metabolic activity in plasma and are of potential use in a liver support bioreactor.
血浆凝血的接触激活可能会限制某些微载体类型在肝辅助装置中用于肝细胞附着的应用。研究了七种微载体类型在含有2 - 500单位肝素/毫升的血浆中的激活情况。葡聚糖（Cytodex 1和2）和胶原包被（Cytodex 3）的微载体在2 - 25单位/毫升（Cytodex 1）和2 - 100单位/毫升（Cytodex 2和3）时激活凝血。聚苯乙烯、明胶、玻璃或纤连蛋白包被的聚苯乙烯微载体未激活凝血。与培养基相比，HepG2细胞在血浆中孵育不影响细胞活力，但增加了细胞数量（56.4对65.1×10⁴个细胞；P＜0.05）以及[³H] - 氨基酸掺入蛋白质的量（204913对279624 dpm；P＜0.05）。附着在聚苯乙烯上的细胞表现出时间线性的蛋白质合成、葡萄糖和7 - 乙氧基香豆素代谢。我们得出结论，附着在聚苯乙烯上的肝细胞在血浆中维持活力和代谢活性，在肝支持生物反应器中具有潜在用途。
