Wu Yunhua, Ji Xiaobo, Hu Shengshui
Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Bioelectrochemistry. 2004 Aug;64(1):91-7. doi: 10.1016/j.bioelechem.2004.03.005.
A novel nanoparticle film modified electrode has been constructed using a glassy carbon electrode (GCE) coated with a carbon nanotube-dihexadecylphosphate (DHP) film. This modified electrode exhibits an enhanced effectiveness for the oxidation of azithromycin. A method is also described for the evaluation of azithromycin-bovine serum albumin (BSA) interaction. The electrochemical behavior of azithromycin as well as its interaction with BSA at this nanoparticle film electrode has been investigated by cyclic voltammetry, linear sweep voltammetry, differential pulse voltammetry and chronocoulometry. The binding number and association constant between azithromycin and bovine serum albumin have been obtained.
一种新型的纳米颗粒薄膜修饰电极已通过在玻碳电极（GCE）上涂覆碳纳米管 - 二己基磷酸酯（DHP）薄膜构建而成。该修饰电极对阿奇霉素的氧化表现出增强的效果。还描述了一种评估阿奇霉素与牛血清白蛋白（BSA）相互作用的方法。通过循环伏安法、线性扫描伏安法、差分脉冲伏安法和计时库仑法研究了阿奇霉素在该纳米颗粒薄膜电极上的电化学行为及其与BSA的相互作用。已获得阿奇霉素与牛血清白蛋白之间的结合数和缔合常数。
