Fleshner Monika, Campisi Jay, Amiri Leila, Diamond David M
Department of Integrative Physiology, University of Colorado--Boulder, Boulder, CO 80309, USA.
Psychoneuroendocrinology. 2004 Oct;29(9):1142-52. doi: 10.1016/j.psyneuen.2004.01.007.
Heat-shock proteins (Hsp) play an important role in stress physiology. Exposure to a variety of stressors will induce intracellular Hsp72, and this induction is believed to be beneficial for cell survival. In contrast, Hsp72 released during stress (extracellular Hsp72; eHsp72) activates pro-inflammatory responses. Clearly, physical stressors such as heat, cold, H(2)O(2), intense exercise and tail shock will induce both intra- and extracellular Hsp72. The current study tested whether a psychological stressor, cat exposure, would also trigger this response. In addition, the potential role of adrenal hormones in the Hsp72 response was examined. Adult, male Sprague Dawley rats were either adrenalectomized (ADX) or sham operated. Ten days post-recovery, rats were exposed to either a cat with no physical contact or control procedures (n = 5-6/group) for 2 h. Levels of intracellular Hsp72 were measured in the brain (frontal cortex, hippocampus, hypothalamus, dorsal vagal complex) and pituitary (ELISA). Levels of eHsp72 (ELISA) and corticosterone (RIA) were measured from serum obtained at the end of the 2-h stress period. Rats that were exposed to a cat had elevated intracellular Hsp72 in hypothalamus and dorsal vagal complex, and elevated eHsp72 and corticosterone in serum. Both the intra- and extracellular Hsp72 responses were blocked or attenuated by ADX. This study demonstrates that cat exposure can stimulate the Hsp72 response and that adrenal hormones contribute to this response.
热休克蛋白（Hsp）在应激生理学中发挥着重要作用。暴露于多种应激源会诱导细胞内Hsp72的产生，并且这种诱导被认为对细胞存活有益。相反，应激期间释放的Hsp72（细胞外Hsp72；eHsp72）会激活促炎反应。显然，诸如热、冷、H₂O₂、剧烈运动和尾部电击等物理应激源会诱导细胞内和细胞外Hsp72的产生。当前的研究测试了一种心理应激源——猫暴露——是否也会引发这种反应。此外，还研究了肾上腺激素在Hsp72反应中的潜在作用。成年雄性Sprague Dawley大鼠要么接受肾上腺切除术（ADX），要么接受假手术。恢复10天后，将大鼠暴露于无身体接触的猫或对照程序下2小时（每组n = 5 - 6只）。通过酶联免疫吸附测定法（ELISA）测量大脑（额叶皮质、海马体、下丘脑、迷走背核复合体）和垂体中的细胞内Hsp72水平。在2小时应激期结束时采集的血清中测量eHsp72（ELISA）和皮质酮（放射免疫分析法，RIA）的水平。暴露于猫的大鼠下丘脑和迷走背核复合体中的细胞内Hsp72升高，血清中的eHsp72和皮质酮升高。ADX阻断或减弱了细胞内和细胞外Hsp72的反应。这项研究表明，猫暴露可以刺激Hsp72反应，并且肾上腺激素促成了这种反应。
