蛋白质对培养的牛动脉内皮细胞单层通透性的影响。

Effects of proteins on the permeability of monolayers of cultured bovine arterial endothelium.

作者信息

Turner M R

机构信息

Department of Physiology and Biophysics, St Mary's Hospital Medical School, London.

出版信息

J Physiol. 1992 Apr;449:21-35. doi: 10.1113/jphysiol.1992.sp019072.

DOI:10.1113/jphysiol.1992.sp019072

PMID:1522510

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1176065/

Abstract

Monolayers of arterial endothelium on porous membranes were exposed to a pressure of 15 cmH2O at 37 degrees C, or of 30 cmH2O at 0 degree C. At constant pressure, the rate of liquid flow per unit area (Jv/A) through each monolayer decreased with time, in the way previously described for cultured endothelium. This phenomenon has been called sealing. After Jv/A stabilized, the pressure was reduced and the hydraulic permeability (Lp) of the endothelium was calculated from the relationship between Jv/A and pressure. Endothelium was seen to be damaged after some experiments at 37 degrees C, but appeared undamaged after experiments at 0 degree C. 2. Bovine serum albumin (BSA) did not influence the Lp of cultured endothelium. At 37 degrees C, the mean (+/- S.E.M.) endothelial Lp was 47.2 +/- 7.3 x 10(-7) cm s-1 cmH2O-1 (n = 10) in the presence of BSA (5 g (100 ml)-1). This is not significantly different from the mean (+/- S.E.M.) Lp of 53.4 +/- 9.0 x 10(-7) cm s-1 cmH2O-1 (n = 9) in the absence of added protein (P greater than 0.10). At 0 degree C also, there was no significant difference between mean Lps in the presence of BSA (0.1 g (100 ml)-1) and in the absence of added protein. 3. Solutions of BSA (5 g (100 ml)-1 or of the neutral polymer Ficoll 70 (4 g (100 ml)-1) did not exert any effective osmotic pressure across endothelium at 37 or 0 degrees C, respectively. 4. BSA (0.1 g (100 ml)-1) did not enable solutions of Ficoll 70 (4 g (100 ml)-1) to exert an effective osmotic pressure across endothelium at 0 degree C. 5. The mean Lp of endothelium at 0 degree C was significantly lower in the presence of cationized ferritin (CF; 0.1 g (100 ml)-1) than in the absence of added protein (P less than 0.001). Native ferritin (NF; 0.1 g (100 ml)-1) had no effect on Lp. 6. In the presence of CF (0.1 g (100 ml)-1), solutions of Ficoll 70 (4 g (100 ml)-1) exerted a mean effective osmotic pressure of 27.7 cmH2O (n = 5) across endothelium at 0 degree C. The mean effective osmotic pressure exerted across endothelium by solutions of Ficoll 70 (4 g (100 ml)-1) plus NF (0.1 g (100 ml)-1) was 1.2 cmH2O (n = 4).

摘要

将多孔膜上的单层动脉内皮细胞在37℃下暴露于15 cmH₂O的压力，或在0℃下暴露于30 cmH₂O的压力。在恒定压力下，通过每个单层的单位面积液体流速（Jv/A）随时间下降，其方式与先前描述的培养内皮细胞相同。这种现象被称为封闭。在Jv/A稳定后，降低压力，并根据Jv/A与压力之间的关系计算内皮细胞的水力传导率（Lp）。在37℃下进行一些实验后，内皮细胞可见受损，但在0℃下进行实验后则未受损。2. 牛血清白蛋白（BSA）不影响培养内皮细胞的Lp。在37℃时，存在BSA（5 g（100 ml）⁻¹）时，内皮细胞Lp的平均值（±标准误）为47.2±7.3×10⁻⁷ cm s⁻¹ cmH₂O⁻¹（n = 10）。这与未添加蛋白质时Lp的平均值（±标准误）53.4±9.0×10⁻⁷ cm s⁻¹ cmH₂O⁻¹（n = 9）无显著差异（P＞0.10）。在0℃时，存在BSA（0.1 g（100 ml）⁻¹）时的平均Lp与未添加蛋白质时也无显著差异。3. BSA溶液（5 g（100 ml）⁻¹）或中性聚合物Ficoll 70溶液（4 g（100 ml）⁻¹）在37℃或0℃时分别不会在内皮细胞上施加任何有效渗透压。4. BSA（0.1 g（100 ml）⁻¹）不能使Ficoll 70溶液（4 g（100 ml）⁻¹）在0℃时在内皮细胞上施加有效渗透压。5. 在存在阳离子化铁蛋白（CF；0.1 g（100 ml）⁻¹）时，0℃下内皮细胞的平均Lp显著低于未添加蛋白质时（P＜0.001）。天然铁蛋白（NF；0.1 g（100 ml）⁻¹）对Lp无影响。6. 在存在CF（0.1 g（100 ml）⁻¹）时，Ficoll 70溶液（4 g（100 ml）⁻¹）在0℃时在内皮细胞上施加的平均有效渗透压为27.7 cmH₂O（n = 5）。Ficoll 70溶液（4 g（100 ml）⁻¹）加NF（0.1 g（100 ml）⁻¹）在内皮细胞上施加的平均有效渗透压为1.2 cmH₂O（n = 4）。