Chang Ya-Wen, Howard Susie C, Herman Paul K
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
Mol Cell. 2004 Jul 2;15(1):107-16. doi: 10.1016/j.molcel.2004.05.021.
RNA polymerase II transcription is a complex process that is controlled at multiple levels. The data presented here add to this repertoire by showing that signal transduction pathways can directly regulate gene expression by targeting components of the general RNA polymerase II apparatus. In particular, this study shows that the Ras/PKA signaling pathway in Saccharomyces cerevisiae regulates the activity of the Srb complex, a regulatory group of proteins that is part of the RNA polymerase II holoenzyme. Genetic and biochemical data indicate that Srb9p is a substrate for PKA and that this phosphorylation modulates the activity of the Srb complex. The Srb complex, like many components of the RNA II polymerase machinery, is responsible for regulating the expression of a relatively large number of genes. Thus, this type of a transcriptional control mechanism would provide the cell with an efficient way of bringing about broad changes in gene expression.
RNA聚合酶II转录是一个在多个水平受到调控的复杂过程。此处呈现的数据通过表明信号转导通路可通过靶向通用RNA聚合酶II装置的组分来直接调节基因表达，从而丰富了这一知识体系。特别地，本研究表明酿酒酵母中的Ras/PKA信号通路调节Srb复合体的活性，Srb复合体是一组调节蛋白，属于RNA聚合酶II全酶的一部分。遗传和生化数据表明Srb9p是PKA的底物，这种磷酸化作用调节了Srb复合体的活性。与RNA II聚合酶机制的许多组分一样，Srb复合体负责调节相对大量基因的表达。因此，这种类型的转录控制机制将为细胞提供一种实现基因表达广泛变化的有效方式。
