College of Horticulture, Xinyang Agriculture and Forestry University, Xinyang 464000, China.
Genes (Basel). 2024 May 10;15(5):608. doi: 10.3390/genes15050608.
var. is a succulent plant with ornamental value. The white-green leaf mutant () showed a significant difference in leaf color from the wild-type plant (WT). In this study, we integrated the transcriptomes of and WT plants to screen differentially expressed genes related to leaf color variation. The results of transcriptome analysis showed that 84,163 unigenes were obtained after de novo assembly and the NR database annotated the largest number of unigenes, which accounted for 57.13%, followed by NT (43.02%), GO (39.84%), Swiss-Prot (39.25%), KEGG (36.06%), and COG (24.88%). Our finding showed that 2586 genes were differentially expressed in the two samples, including 1996 down-regulated genes and 590 up-regulated genes. GO analysis predicted that these differentially expressed genes (DEGs) participate in 12 cellular components, 20 biological processes, and 13 molecular function terms and KEGG analysis showed that metabolic pathways, plant-pathogen interaction, glycerophospholipid metabolism, endocytosis, plant hormone signal transduction, and ether lipid metabolism were enriched among all identified pathways. Through functional enrichment analysis of DEGs, we found that they were involved in chloroplast division and the biosynthesis of plant pigments, including chlorophyll, carotenoids, anthocyanin, and transcription factor families, which might be related to the formation mechanism of leaf color. Taken together, these results present insights into the difference in gene expression characteristics in leaves between WT and mutants and provide a new insight for breeding colorful leaf phenotypes in succulent plants.
变异体是一种具有观赏价值的肉质植物。白色-绿色叶片突变体（）与野生型植物（WT）在叶片颜色上表现出显著差异。在这项研究中，我们整合了和 WT 植物的转录组，以筛选与叶片颜色变化相关的差异表达基因。转录组分析的结果表明，经从头组装获得了 84163 条 unigenes，其中 NR 数据库注释的 unigenes数量最多，占 57.13%，其次是 NT（43.02%）、GO（39.84%）、Swiss-Prot（39.25%）、KEGG（36.06%）和 COG（24.88%）。我们的研究结果表明，在这两个样本中，有 2586 个基因差异表达，包括 1996 个下调基因和 590 个上调基因。GO 分析预测，这些差异表达基因（DEGs）参与了 12 个细胞成分、20 个生物过程和 13 个分子功能术语，KEGG 分析表明，代谢途径、植物-病原体相互作用、甘油磷脂代谢、内吞作用、植物激素信号转导和醚脂代谢在所有鉴定的途径中都得到了富集。通过 DEGs 的功能富集分析，我们发现它们参与了叶绿体分裂和植物色素的生物合成，包括叶绿素、类胡萝卜素、花青素和转录因子家族，这可能与叶片颜色的形成机制有关。总之，这些结果揭示了 WT 和 突变体叶片之间基因表达特征的差异，为肉质植物彩色叶片表型的培育提供了新的见解。
