酵母GPX2编码磷脂氢过氧化物谷胱甘肽过氧化物酶受氧化应激和钙调神经磷酸酶/Crz1介导的Ca2+信号通路的独特调控机制。

Distinct regulatory mechanism of yeast GPX2 encoding phospholipid hydroperoxide glutathione peroxidase by oxidative stress and a calcineurin/Crz1-mediated Ca2+ signaling pathway.

作者信息

Tsuzi Daisuke, Maeta Kazuhiro, Takatsume Yoshifumi, Izawa Shingo, Inoue Yoshiharu

机构信息

Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.

出版信息

FEBS Lett. 2004 Jul 2;569(1-3):301-6. doi: 10.1016/j.febslet.2004.05.077.

DOI:10.1016/j.febslet.2004.05.077

PMID:15225652

Abstract

The GPX2 gene encodes a homologue of mammalian phospholipid hydroperoxide glutathione peroxidase in Saccharomyces cerevisiae. Previously, we have reported that the oxidative stress-induced expression of GPX2 is strictly regulated by Yap1 and Skn7 transcription factors. Here, we found that the expression of GPX2 is induced by CaCl(2) in a calcineurin (CN)/Crz1-dependent manner, and the CN-dependent response element was specified in the GPX2 promoter. Neither Yap1 nor Skn7 was required for Ca(2+)-dependent induction of GPX2, therefore, distinct regulation for the oxidative stress response and Ca(2+) signal response for GPX2 exists in yeast cells.

摘要

GPX2基因在酿酒酵母中编码哺乳动物磷脂氢过氧化物谷胱甘肽过氧化物酶的同源物。此前，我们曾报道过，GPX2的氧化应激诱导表达受到Yap1和Skn7转录因子的严格调控。在此，我们发现，GPX2的表达由CaCl₂以钙调神经磷酸酶（CN）/Crz1依赖的方式诱导，且在GPX2启动子中确定了CN依赖的反应元件。Ca²⁺依赖的GPX2诱导不需要Yap1和Skn7，因此，酵母细胞中存在对GPX2氧化应激反应和Ca²⁺信号反应的不同调控。