Bryant-Friedrich Amanda C
Department of Chemistry, Oakland University, Rochester, Michigan 48309, USA.
Org Lett. 2004 Jul 8;6(14):2329-32. doi: 10.1021/ol0493453.
[reaction: see text] A C-3'-thymidinyl radical has been photochemically generated site-specifically in DNA oligonucleotides. A nucleoside H-phosphonate bearing a C-3' acetyl group was incorporated into DNA oligomers using a hand-coupling technique. When nucleotides containing the modified monomer were photolyzed (> or =320 nm) in the presence of a hydrogen atom donor, reduction products were detected by RP-HPLC and MALDI-ToF MS analysis.
[反应：见正文] C-3'-胸苷基自由基已在DNA寡核苷酸中通过光化学方法位点特异性生成。使用手动偶联技术将带有C-3'乙酰基的核苷H-膦酸酯掺入DNA寡聚物中。当含有修饰单体的核苷酸在氢原子供体存在下进行光解（≥320 nm）时，通过反相高效液相色谱（RP-HPLC）和基质辅助激光解吸电离飞行时间质谱（MALDI-ToF MS）分析检测到还原产物。
