Sykes Jane E, Bailiff Nathan L, Ball Louise M, Foreman Oded, George Jeanne W, Fry Michael M
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
J Am Vet Med Assoc. 2004 Jun 15;224(12):1946-51, 1930-1. doi: 10.2460/javma.2004.224.1946.
A 3-year-old sexually intact male Bull Mastiff underwent splenectomy for splenic thrombosis; prior to and after splenectomy, multiple blood transfusions were administered. Two weeks after the procedure, T-cell lymphoproliferative disease was diagnosed. Treatment with prednisone and chlorambucil was initiated, and 2 weeks later, cytologic examination of a blood smear revealed small (0.3 microm), coccoid basophilic bodies on the surface of approximately 70% of the RBCs. Morphologically, these resembled "Candidatus Mycoplasma haemominutum." A polymerase chain reaction assay was used to amplify a partial 16S rRNA sequence in blood obtained from the dog; the product was sequenced and compared with 16S rRNA gene sequences of other hemotropic mycoplasmas. The sequence was 98% homologous to that of "Candidatus M haemominutum", but only 77% homologous to that of M haemocanis and M haemofelis.
一只3岁未绝育的雄性斗牛獒因脾血栓形成接受了脾切除术；在脾切除术前和术后，进行了多次输血。术后两周，诊断出T细胞淋巴增殖性疾病。开始使用泼尼松和苯丁酸氮芥进行治疗，两周后，血液涂片的细胞学检查显示，约70%的红细胞表面有小的（0.3微米）、球形嗜碱性小体。形态学上，这些类似于“嗜吞噬细胞无形体”。使用聚合酶链反应分析法扩增从该犬采集的血液中的部分16S rRNA序列；对产物进行测序，并与其他血源性支原体的16S rRNA基因序列进行比较。该序列与“嗜吞噬细胞无形体”的序列同源性为98%，但与犬血支原体和猫血支原体的序列同源性仅为77%。
