Malinowski E, Smulski S, Markiewicz H, Kaczmarowski M
Department of Pathophysiology of Reproduction and Mammary Gland, National Veterinary Research Institute, Powstańców Wlkp. 10, 85-090 Bydgoszcz, Poland.
Pol J Vet Sci. 2004;7(2):91-5.
The purpose of the trial was to establish the effect of the injection of the lysozyme dimer or vitamins connected with Se on the activity of chosen antioxidant enzymes and the total antioxidant status in pregnant heifers. Examinations were carried out during winter season in one farm on 21 heifers aged 22-24 months. Between the 21st and 14st day before expected parturition, seven heifers were once i.m. injected with antioxidants (Vitamin A-600 000 i.u.; Vitamin D3-200 000 i.u.; Vitamin E-1.5 mg/kg b.w., Selenium-0.022 mg/kg b.w.), and the next seven animals with lysozyme dimer (Lydium-KLP) at a dose of 0.02 mg/kg b.w. versus 7 non-treated control animals. Blood samples were taken before injection and then in hour 24 and 72 after injection, and between, the 7th and 14th day after calving. The activity of superoxide dismutase (SOD), glutathione peroxidase (GSHpx), glutathione reductase (GSHred) and total antioxidant status (TAS) were measured by colorimetric method with the use of Randox kits. The mean value of SOD activity 21-14 days before expected calving was 704.8 +/- 294.6 U/ml of whole blood, GSHpx 59222 +/- 23699 U/l of whole blood, GSHred 110.8 +/- 22.5 U/l and TAS 0.33 +/- 0.15 mmol/l of serum. These indicators did not change in the control group with the exception of a statistically insignificant decrease in SOD activity after parturition. Statistically significant increase in blood SOD activity was noted only in the first day after injection of vitamins combined with selenium. These antioxidants also caused an insignificant increase in blood GSHpx activity in 72 hour following the injection, and in the second week after calving (statistically significant). The injection of antioxidants or lysozyme dimer did not change the activity of blood GSHred. However, an increase in the TAS was found in hour 24 (non significant) and 72 (statistically significant) following the single injection of lysozyme dimer.
该试验的目的是确定注射溶菌酶二聚体或与硒相关的维生素对怀孕小母牛体内所选抗氧化酶活性及总抗氧化状态的影响。在冬季，于一个农场对21头22 - 24月龄的小母牛进行了检测。在预计分娩前第21天至第14天期间，7头小母牛一次肌肉注射抗氧化剂（维生素A - 600000国际单位；维生素D3 - 200000国际单位；维生素E - 1.5毫克/千克体重，硒 - 0.022毫克/千克体重），另外7头动物注射剂量为0.02毫克/千克体重的溶菌酶二聚体（Lydium - KLP），还有7头为未处理的对照动物。在注射前、注射后24小时和72小时以及产犊后第7天至第14天采集血样。使用朗道试剂盒通过比色法测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHpx）、谷胱甘肽还原酶（GSHred）的活性及总抗氧化状态（TAS）。预计产犊前21 - 14天，全血中SOD活性的平均值为704.8±294.6单位/毫升，GSHpx为59222±23699单位/升全血，GSHred为110.8±22.5单位/升，血清TAS为0.33±0.15毫摩尔/升。对照组中这些指标除产后SOD活性有统计学上无显著意义的下降外均未改变。仅在注射维生素与硒的组合后的第一天，血中SOD活性有统计学上显著的升高。这些抗氧化剂在注射后72小时及产犊后第二周还使血中GSHpx活性有不显著的升高（统计学上显著）。注射抗氧化剂或溶菌酶二聚体并未改变血中GSHred的活性。然而，单次注射溶菌酶二聚体后24小时（无显著意义）和72小时（统计学上显著）TAS有所升高。
