Tagg J R
Department of Microbiology, University of Otago, P.O. Box 56, Dunedin, New Zealand.
Indian J Med Res. 2004 May;119 Suppl:13-6.
BACKGROUND & OBJECTIVES: Streptococcus salivarius is a numerically prominent member of the human oral microbiota that produces a variety of bacteriocin-like inhibitory substances (BLIS) having in vitro inhibitory activity against S. pyogenes. Our previous studies of S. salivarius isolates from children using a deferred antagonism BLIS production (P)-typing scheme showed that the 9 per cent of children having large populations of P-type 677 S. salivarius experienced fewer S. pyogenes acquisitions than either the 11 per cent of children having predominant P-type 226 populations or the 60 per cent of children with largely non-inhibitory (P-type 000) S. salivarius. Amongst the other BLIS P-types detected were a number of strongly-inhibitory (P-type 777) S. salivarius. In the present study the inhibitory agents produced by prototype strains of P-types 226, 677 and 777 S. salivarius are compared.
The prototype BLIS-producing S. salivarius strains SN, 20P3, and K12 were isolated from tongue swabbings. BLIS P-typing was done using standard procedures. The BLIS molecules were purified and characterized.
S. salivarius SN (P-type 226) produces a heat-labile muramidase. S. salivarius 20P3 (P-type 677) produces the 2315 Da lantibiotic salivaricin A and S. salivarius K12 (P-type 777) produces two lantibiotics; salivaricin A2 (2368 Da) and salivaricin B (2733 Da).
INTERPRETATION & CONCLUSION: The P-type 777 S. salivarius strain produced salivaricin A2 and salivaricin B. The combined production of two anti-S. pyogenes BLIS activities by this strain indicates that it could be adopted as a colonizing strain in bacterial interference trials.
唾液链球菌是人类口腔微生物群中数量上占突出地位的成员，可产生多种类细菌素抑制物质（BLIS），对化脓性链球菌具有体外抑制活性。我们之前采用延迟拮抗BLIS产生（P）分型方案对儿童分离出的唾液链球菌进行研究，结果显示，9%唾液链球菌P型677数量多的儿童，其化脓性链球菌感染发生率低于11%唾液链球菌P型226占主导的儿童，也低于60%唾液链球菌基本无抑制作用（P型000）的儿童。在检测到的其他BLIS P型中，有一些是强抑制性（P型777）唾液链球菌。在本研究中，对P型226、677和777唾液链球菌的原型菌株产生的抑制剂进行了比较。
从舌拭子中分离出产生BLIS的唾液链球菌原型菌株SN、20P3和K12。采用标准程序进行BLIS P分型。对BLIS分子进行纯化和表征。
唾液链球菌SN（P型226）产生一种热不稳定的胞壁质酶。唾液链球菌20P3（P型677）产生2315 Da的羊毛硫抗生素唾液乳链菌肽A，唾液链球菌K12（P型777）产生两种羊毛硫抗生素；唾液乳链菌肽A2（2368 Da）和唾液乳链菌肽B（2733 Da）。
P型777唾液链球菌菌株产生唾液乳链菌肽A2和唾液乳链菌肽B。该菌株联合产生两种抗化脓性链球菌的BLIS活性，表明它可作为细菌干扰试验中的定植菌株。
