Mauriello G, Ercolini D, La Storia A, Casaburi A, Villani F
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, Naples, Italy.
J Appl Microbiol. 2004;97(2):314-22. doi: 10.1111/j.1365-2672.2004.02299.x.
The aims of this work were to (i) use a bacteriocin produced by Lactobacillus curvatus 32Y active against Listeria monocytogenes to activate polythene films by different methods, (ii) implement a large-scale process for antilisterial polythene films production and (iii) verify the efficacy of the developed films in inhibiting the growth of L. monocytogenes during the storage of meat products.
The film was made active by using the antilisterial bacteriocin 32Y by Lact. curvatus with three different procedures: soaking, spraying and coating. The antimicrobial activity of the activated films was tested in plate assays against the indicator strain L. monocytogenes V7. All the used procedures yielded active polythene films although the quality of the inhibition was different. The coating was therefore employed to develop active polythene films in an industrial plant. The antimicrobial activity of the industrially produced films was tested in experiments of food packaging involving pork steak and ground beef contaminated by L. monocytogenes V7 at roughly 10(3) CFU cm(-2) and gram respectively. The results of the challenge tests showed the highest antimicrobial activity after 24 h at 4 degrees C, with a decrease of about 1 log of the L. monocytogenes population.
Antimicrobial packaging can play an important role in reducing the risk of pathogen development, as well as extending the shelf life of foods.
Studies of new food-grade bacteriocins as preservatives and development of suitable systems of bacteriocin treatment of plastic films for food packaging are important issues in applied microbiology and biotechnology, both for implementing and improving effective hurdle technologies for a better preservation of food products.
本研究的目的是：（i）使用弯曲乳杆菌32Y产生的对单核细胞增生李斯特菌有活性的细菌素，通过不同方法使聚乙烯薄膜具有活性；（ii）实施大规模生产抗李斯特菌聚乙烯薄膜的工艺；（iii）验证所制备薄膜在肉类产品储存期间抑制单核细胞增生李斯特菌生长的功效。
使用弯曲乳杆菌产生的抗李斯特菌细菌素32Y，通过三种不同程序使薄膜具有活性：浸泡、喷涂和涂布。在平板试验中针对指示菌株单核细胞增生李斯特菌V7测试活化薄膜的抗菌活性。尽管抑制质量不同，但所有使用的程序均产生了具有活性的聚乙烯薄膜。因此，采用涂布法在工业工厂中制备活性聚乙烯薄膜。在涉及被单核细胞增生李斯特菌V7污染的猪排和碎牛肉的食品包装实验中，测试工业生产薄膜的抗菌活性，污染程度分别约为每平方厘米10³CFU和每克10³CFU。挑战试验结果表明，在4℃下放置24小时后抗菌活性最高，单核细胞增生李斯特菌数量减少约1个对数级。
抗菌包装在降低病原体滋生风险以及延长食品保质期方面可发挥重要作用。
研究新型食品级细菌素作为防腐剂以及开发适用于食品包装塑料薄膜的细菌素处理系统，是应用微生物学和生物技术中的重要问题，对于实施和改进有效的栅栏技术以更好地保存食品产品而言均是如此。
