Maky Mohamed Abdelfattah, Sonomoto Kenji, Zendo Takeshi
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Department of Food Hygiene and Control, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt.
Int J Mol Sci. 2025 Jun 25;26(13):6083. doi: 10.3390/ijms26136083.
Antimicrobial food packaging is considered a promising technology to improve food safety by inhibiting or reducing the growth of food microorganisms and minimizing the need for preservatives. This study aimed to develop and evaluate carboxymethyl cellulose (CMC) films integrated with bacteriocins for antibacterial efficacy. Plantaricin W was assessed as a potential bacteriocin for activation of CMC to control the dangerous food-borne pathogen, . Minced beef samples were inoculated with ATCC BAA-679 and treated with plantaricin W-activated food packaging. The results showed a significant reduction of the target pathogen by approximately 1 log cycle compared to the control group. Enterocin F4-9 is a novel bacteriocin that acts on Gram-negative microbes that were not affected by plantaricin W. Therefore, a novel food packaging activated with plantaricin W and enterocin F4-9 was developed to broaden their antimicrobial activity. The effect of this film on meat-associated microbes was investigated. The results demonstrated that the film significantly reduced the counts of mesophilic and psychotropic bacteria by 86.67% and 96.67%, respectively. Additionally, the pH values of the treated meat samples were significantly lower than those of the untreated controls. The obtained findings indicated that bacteriocin-activated CMC films could potentially be utilized as antimicrobial packaging in modern food technology.
抗菌食品包装被认为是一种很有前景的技术，可通过抑制或减少食品微生物的生长以及尽量减少防腐剂的使用来提高食品安全。本研究旨在开发和评估整合了细菌素的羧甲基纤维素（CMC）薄膜的抗菌效果。评估了植物乳杆菌素W作为激活CMC以控制危险食源性病原体的潜在细菌素。将碎牛肉样品接种ATCC BAA - 679，并用植物乳杆菌素W激活的食品包装进行处理。结果显示，与对照组相比，目标病原体显著减少了约1个对数周期。肠球菌素F4 - 9是一种新型细菌素，作用于不受植物乳杆菌素W影响的革兰氏阴性微生物。因此，开发了一种用植物乳杆菌素W和肠球菌素F4 - 9激活的新型食品包装，以扩大其抗菌活性。研究了这种薄膜对与肉类相关的微生物的影响。结果表明，该薄膜使嗜温菌和嗜冷菌的数量分别显著减少了86.67%和96.67%。此外，处理过的肉类样品的pH值显著低于未处理的对照组。所得结果表明，细菌素激活的CMC薄膜在现代食品技术中有可能用作抗菌包装。
