Konings Frank A J, Nyambi Phillipe N
Department of Microbiology, New York University School of Medicine, 10010, USA.
AIDS Res Hum Retroviruses. 2004 Jun;20(6):673-8. doi: 10.1089/0889222041217464.
We describe the resistance-associated substitutions that are present in the reverse transcriptase (RT) genes of HIV-1 CRF02_AG strains infecting drug-naive villagers of Cameroon. The 11 sequences analyzed were previously subtyped as CRF02_AG in the gag, pro, and env genes, and this work revealed that most (10/11) had a concordant subtype (CRF02_AG) in the pol gene, while one sequence had discordant subtype (A1) in the pol gene. Classification of the CRF02_AG sequences was further confirmed by recombination breakpoint analysis, which revealed a mosaic composition similar to the reference strain IbNG. Analysis of the RT genes for resistance-associated substitutions revealed two sequences containing a V118I substitution. Even though no other resistance-associated substitutions were found, the presence of V118I, which is implicated in resistance to reverse transcriptase inhibitors, in CRF02_AG strains infecting drug-naive individuals should be considered when introducing these antiretrovirals in areas where CRF02_AG is the predominant subtype, such as Cameroon.
我们描述了感染喀麦隆未接受过药物治疗的村民的HIV-1 CRF02_AG毒株逆转录酶（RT）基因中存在的与耐药相关的替换。分析的11个序列先前在gag、pro和env基因中被亚型化为CRF02_AG，这项研究表明，大多数（10/11）在pol基因中具有一致的亚型（CRF02_AG），而一个序列在pol基因中具有不一致的亚型（A1）。通过重组断点分析进一步证实了CRF02_AG序列的分类，该分析揭示了与参考毒株IbNG相似的嵌合组成。对RT基因进行与耐药相关替换的分析发现，有两个序列含有V118I替换。尽管未发现其他与耐药相关的替换，但在将这些抗逆转录病毒药物引入CRF02_AG为主要亚型的地区（如喀麦隆）时，应考虑在感染未接受过药物治疗个体的CRF02_AG毒株中存在与逆转录酶抑制剂耐药相关的V118I。
