Kumar M Palani, Vairamani M, Raju Raju Prasada, Lobo Charmaine, Anbumani N, Kumar C P Girish, Menon Thangam, Shanmugasundaram S
Department of Microbiology, Dr ALM Postgraduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Indian J Med Res. 2004 Jun;119(6):283-8.
BACKGROUND & OBJECTIVES: Beta haemolytic streptococci belonging to Lancefield group A, B, C and G cause a wide spectrum of clinical diseases. Hence there is a need for rapid and accurate typing of these strains. The present study was undertaken to evaluate the use of intact cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for rapid discrimination between strains of beta haemolytic streptococci.
Colonies of beta haemolytic streptococci were emulsified with chemical matrix on the sample slide, dried and analyzed by MALDI-TOF-MS.
The reproducibility of results for all groups of beta haemolytic streptococci was good and spectra obtained for Lancefield group A, C and G streptococci showed discrimination between the groups on visual comparison. A finer difference in spectrum was observed among group A streptococci isolated from different locations at different periods of time.
INTERPRETATION & CONCLUSION: MALDI-TOF-MS may be a potential tool in discriminating between strains of beta haemolytic streptococci, and also in the characterisation of untypable strains of group A streptococci.
属于A、B、C和G Lancefield组的β溶血性链球菌可引发多种临床疾病。因此，需要对这些菌株进行快速、准确的分型。本研究旨在评估完整细胞基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）在快速鉴别β溶血性链球菌菌株方面的应用。
将β溶血性链球菌菌落与化学基质在载玻片上乳化，干燥后用MALDI-TOF-MS进行分析。
所有β溶血性链球菌组的结果重现性良好，A、C和G Lancefield组链球菌获得的质谱在视觉比较上显示出组间差异。在不同时间从不同地点分离的A组链球菌之间观察到更细微的光谱差异。
MALDI-TOF-MS可能是鉴别β溶血性链球菌菌株以及对A组链球菌不可分型菌株进行特征分析的潜在工具。
