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人肝癌细胞系转移相关蛋白的差异蛋白质组学分析

Differential proteomic analysis of human hepatocellular carcinoma cell line metastasis-associated proteins.

作者信息

Cui Jie-Feng, Liu Yin-Kun, Pan Bo-Shen, Song Hai-Yan, Zhang Yu, Sun Rui-Xia, Chen Jie, Feng Ju-Tao, Tang Zhao-You, Yu Yan-Ling, Shen Hua-Li, Yang Peng-Yuan

机构信息

Liver Cancer Institute, Zhongshan Hospital, Fudan University, 136 Yi Xue Yuan Road, 200032 Shanghai, China.

出版信息

J Cancer Res Clin Oncol. 2004 Oct;130(10):615-22. doi: 10.1007/s00432-004-0576-5. Epub 2004 Jul 9.

DOI:10.1007/s00432-004-0576-5
PMID:15243804
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12161779/
Abstract

PURPOSE

The comparative study of differentially expression of protein profiles of hepatocellular carcinoma cell lines with various metastasic potential and screening key molecules related to hepatocellular carcinoma metastasis and recurrence.

METHODS

Using two-dimensional electrophoresis and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), we analyzed differentially displayed proteomics of human hepatocellular carcinoma cell lines Hep3B, MHCC97L, MHCC97H with different metastasic potential.

RESULTS

Approximate 1,000 protein spots were detected on silver-stained gel by ImageMaster (977+/-113 spots in Hep3B, 1092+/-40 in MHCC97L, and 889+/-14 in MHCC97H). Fifty distinct different protein spots were analyzed with online LC-ESI-MS/MS. Only 26 protein spots had a positive result, including annexin1, S100A4, and so on. In comparison with nonmetastasis Hep3B cell lines, there were 16 proteins overexpressed in MHCC97H and MHCC97L, 10 proteins underexpressed in MHCC97H and MHCC97L. Applying cell immunohistochemistry and RT-PCR, we further validated two interesting and different proteins, annexin1 and S100A4.

CONCLUSION

The protein profile of metastatic hepatocellular carcinoma cell lines displayed obvious differences compared with non-metastatic liver cancer cell lines. The results imply that various different proteins may lead to HCC metastasis together.

摘要

目的

比较不同转移潜能的肝癌细胞系蛋白质谱的差异表达，筛选与肝癌转移和复发相关的关键分子。

方法

采用二维电泳和液相色谱 - 电喷雾电离 - 串联质谱（LC - ESI - MS/MS）技术，分析人肝癌细胞系Hep3B、MHCC97L、MHCC97H不同转移潜能的差异蛋白质组学。

结果

通过ImageMaster在银染凝胶上检测到约1000个蛋白质点（Hep3B中977±113个点，MHCC97L中1092±40个点，MHCC97H中889±14个点）。用在线LC - ESI - MS/MS分析了50个不同的蛋白质点。仅26个蛋白质点有阳性结果，包括膜联蛋白1、S100A4等。与非转移的Hep3B细胞系相比，MHCC97H和MHCC97L中有16种蛋白质过表达，10种蛋白质低表达。应用细胞免疫组化和RT - PCR，进一步验证了两种有趣且不同的蛋白质，膜联蛋白1和S100A4。

结论

转移性肝癌细胞系的蛋白质谱与非转移性肝癌细胞系相比有明显差异。结果提示多种不同蛋白质可能共同导致肝癌转移。

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