Pedersen K B, Nesland J M, Fodstad Ø, Maelandsmo G M
Department of Tumour Biology, The Norwegian Radium Hospital, Montebello, N-0310 Oslo, Norway.
Br J Cancer. 2002 Nov 18;87(11):1281-6. doi: 10.1038/sj.bjc.6600624.
In 66 breast cancer biopsies, the expression of the Ca(2+)-binding protein S100A4, E-cadherin, alpha- and beta-catenin was examined by immunohistochemistry, and the results were related to clinical and pathological parameters. High levels of S100A4 were found to significantly correlate with histological grade (P=0.030) and loss of oestrogen receptor (P=0.046), but not to the time interval between surgery and development of distant metastasis (P=0.51) or to patient survival (P=0.89). Loss of E-cadherin expression, associated with altered cell-cell adhesion, showed a highly significant association to overall survival (P=0.020) and metastasis-free period (P=0.0052). In multivariate analysis, only lymph node involvement was a more significant predictor of patient demise. No association was found between expression of S100A4 and any single member of the cadherin-catenin complex, but a trend (P=0.053) towards reduced expression of one or several of these proteins and S100A4 immunoreactivity was observed. In conclusion, although our results suggest an association between S100A4 expression and an aggressive tumour phenotype, no relationship to overall survival was found. Deregulation of E-cadherin expression, however, was of high prognostic significance.
在66例乳腺癌活检样本中，通过免疫组织化学检测了钙结合蛋白S100A4、E-钙黏蛋白、α-连环蛋白和β-连环蛋白的表达情况，并将结果与临床和病理参数相关联。结果发现，高水平的S100A4与组织学分级显著相关（P = 0.030）以及雌激素受体缺失相关（P = 0.046），但与手术至远处转移发生的时间间隔无关（P = 0.51），也与患者生存率无关（P = 0.89）。E-钙黏蛋白表达缺失与细胞间黏附改变相关，与总生存率（P = 0.020）和无转移生存期（P = 0.0052）显示出高度显著的相关性。在多变量分析中，只有淋巴结受累是患者死亡的更显著预测因素。未发现S100A4的表达与钙黏蛋白-连环蛋白复合体的任何单个成员之间存在关联，但观察到这些蛋白中的一种或几种的表达降低以及S100A4免疫反应性有下降趋势（P = 0.053）。总之，尽管我们的结果表明S1
