单蕊羊蹄甲叶提取物的抗氧化活性。

Antioxidant activity of leaf extracts from Bauhinia monandra.

作者信息

Argolo A C C, Sant'Ana A E G, Pletsch M, Coelho L C B B

机构信息

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, S/N Cidade Universitária, Pernambuco, Brazil.

出版信息

Bioresour Technol. 2004 Nov;95(2):229-33. doi: 10.1016/j.biortech.2003.12.014.

DOI:10.1016/j.biortech.2003.12.014

PMID:15246449

Abstract

Bauhinia monandra Kurz. is used in Brazil for the treatment of diabetes. Since this activity may be correlated with the presence of antioxidant compounds, leaf extracts of B. monandra were evaluated for their radical scavenging capacity (RSC). An ethanolic extract was taken up in aqueous methanol and partitioned with hexane, chloroform, ethyl acetate to yield three organic extracts together with remaining aqueous extract. The RSC was determined spectrophotometrically using 1,1-diphenylpicrylhydrazyl free radical (DPPH). The chloroform and ethyl acetate extracts were the most appropriate as sources of antioxidant compounds as shown by their inhibition concentration (IC50) and inhibition percentage (IP) values. The antioxidant activity of such extracts was attributed to the presence of three compounds of different polarities (flavonoids and steroids). The chloroform and ethyl acetate extracts exhibited an IC50 of approximately 2 mg/g DPPH and IP values in the range of 60-65%. The results indicate that the extracts of B. monandra have a very potent antioxidant activity, compared with the pure catechins used as positive controls and with other plant extracts.

摘要

单蕊羊蹄甲在巴西被用于治疗糖尿病。由于这种活性可能与抗氧化化合物的存在有关，因此对单蕊羊蹄甲的叶提取物进行了自由基清除能力（RSC）评估。将乙醇提取物溶于甲醇水溶液中，并用己烷、氯仿、乙酸乙酯进行分配，得到三种有机提取物以及剩余的水提取物。使用1,1-二苯基-2-苦基肼自由基（DPPH）通过分光光度法测定RSC。氯仿和乙酸乙酯提取物作为抗氧化化合物的来源最为合适，这从它们的抑制浓度（IC50）和抑制率（IP）值可以看出。此类提取物的抗氧化活性归因于三种不同极性的化合物（黄酮类化合物和甾体）的存在。氯仿和乙酸乙酯提取物的IC50约为2 mg/g DPPH，IP值在60 - 65%范围内。结果表明，与用作阳性对照的纯儿茶素以及其他植物提取物相比，单蕊羊蹄甲的提取物具有非常强的抗氧化活性。