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1
Membrane-associated assembly of M13 phage in extracts of virus-infected Escherichia coli.M13噬菌体在病毒感染的大肠杆菌提取物中的膜相关组装。
Proc Natl Acad Sci U S A. 1977 Feb;74(2):505-9. doi: 10.1073/pnas.74.2.505.
2
Soluble precursor of an integral membrane protein: synthesis of procoat protein in Escherichia coli infected with bacteriophage M13.整合膜蛋白的可溶性前体：感染噬菌体M13的大肠杆菌中前衣壳蛋白的合成。
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1199-203. doi: 10.1073/pnas.76.3.1199.
3
Synthesis of phage M13 specific proteins in a DNA-dependent cell-free system.在依赖DNA的无细胞系统中噬菌体M13特异性蛋白质的合成。
FEBS Lett. 1973 Sep 1;35(1):155-60. doi: 10.1016/0014-5793(73)80600-3.
4
Packaging and maturation of DNA of bacteriophage T7 in vitro.噬菌体T7 DNA在体外的包装与成熟
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3545-9. doi: 10.1073/pnas.71.9.3545.
5
Synthesis of phage M13 coat protein and its assembly into membranes in vitro.噬菌体M13外壳蛋白的体外合成及其在膜中的组装。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1754-8. doi: 10.1073/pnas.75.4.1754.
6
Structure and assembly of bacteriophage PRD1, and Escherichia coli virus with a membrane.噬菌体PRD1的结构与组装，以及一种带有膜的大肠杆菌病毒
J Struct Biol. 1990 Jul-Sep;104(1-3):44-51. doi: 10.1016/1047-8477(90)90056-i.
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Bacterial mutants which block phage assembly.阻断噬菌体组装的细菌突变体。
J Supramol Struct. 1974;2(2-4):349-59. doi: 10.1002/jss.400020224.
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Purification and properties of coliphage 21 repressor.大肠杆菌噬菌体21阻遏物的纯化及特性
Eur J Biochem. 1977 Mar 1;73(2):601-6. doi: 10.1111/j.1432-1033.1977.tb11355.x.
9
phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.体外phiX174依赖DNA的DNA合成：大肠杆菌dnaB突变提取物中P1禁令蛋白的需求
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3907-11. doi: 10.1073/pnas.72.10.3907.
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A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA.噬菌体M13病毒粒子的一种外壳蛋白参与DNA的膜定向合成。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):205-9. doi: 10.1073/pnas.70.1.205.

引用本文的文献

1
Cell-free expression system: a promising platform for bacteriophage production and engineering.无细胞表达系统：噬菌体生产与工程改造的一个有前景的平台。
Microb Cell Fact. 2025 Feb 17;24(1):42. doi: 10.1186/s12934-025-02661-9.
2
Role of M13 gene 1 protein in filamentous virus assembly.M13基因1蛋白在丝状病毒组装中的作用。
J Virol. 1980 Jul;35(1):256-8. doi: 10.1128/JVI.35.1.256-258.1980.
3
The purification of M13 procoat, a membrane protein precursor.M13前衣壳蛋白（一种膜蛋白前体）的纯化
EMBO J. 1982;1(5):573-8. doi: 10.1002/j.1460-2075.1982.tb01210.x.
4
Demonstration by a novel genetic technique that leader peptidase is an essential enzyme of Escherichia coli.一种新型基因技术证明前导肽酶是大肠杆菌的一种必需酶。
J Bacteriol. 1983 Apr;154(1):76-83. doi: 10.1128/jb.154.1.76-83.1983.
5
Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo.大肠杆菌前导肽酶基因的分离及前导肽酶体内过量表达的影响。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6106-10. doi: 10.1073/pnas.78.10.6106.
6
Ff coliphages: structural and functional relationships.丝状大肠杆菌噬菌体：结构与功能关系
Microbiol Rev. 1986 Dec;50(4):401-27. doi: 10.1128/mr.50.4.401-427.1986.
7
Recombinant forms of M13 procoat with an OmpA leader sequence or a large carboxy-terminal extension retain their independence of secY function.带有OmpA前导序列或大型羧基末端延伸的M13原衣壳重组形式保持其对secY功能的独立性。
EMBO J. 1987 Feb;6(2):501-5. doi: 10.1002/j.1460-2075.1987.tb04781.x.
8
Synthesis of phage M13 coat protein and its assembly into membranes in vitro.噬菌体M13外壳蛋白的体外合成及其在膜中的组装。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1754-8. doi: 10.1073/pnas.75.4.1754.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Conditional lethal mutants of the small filamentous coliphage M13. I. Isolation, complementation, cell killing, time of cistron action.小丝状大肠杆菌噬菌体M13的条件致死突变体。I. 分离、互补作用、细胞杀伤、顺反子作用时间
Virology. 1966 Nov;30(3):397-410. doi: 10.1016/0042-6822(66)90118-8.
3
Conditional lethal mutants of the small filamentous coliphage M13. II. Two genes for coat proteins.小丝状大肠杆菌噬菌体M13的条件致死突变体。II. 两种外壳蛋白基因。
Virology. 1969 Sep;39(1):42-53. doi: 10.1016/0042-6822(69)90346-8.
4
[Virus proteins. IV. Constitution of the coat protein of the fd phage].[病毒蛋白。IV. fd噬菌体外壳蛋白的组成]
Hoppe Seylers Z Physiol Chem. 1969 Sep;350(9):1047-66.
5
Filamentous bacterial viruses.丝状细菌病毒。
Bacteriol Rev. 1969 Jun;33(2):172-209. doi: 10.1128/br.33.2.172-209.1969.
6
Genetic control of bacteriophage M13 DNA synthesis.噬菌体M13 DNA合成的遗传控制。
J Mol Biol. 1968 Oct 14;37(1):181-200. doi: 10.1016/0022-2836(68)90082-x.
7
Complex of bacteriophage M13 single-stranded DNA and gene 5 protein.噬菌体M13单链DNA与基因5蛋白复合物
J Mol Biol. 1974 Feb 5;82(4):425-39. doi: 10.1016/0022-2836(74)90239-3.
8
Association of newly synthesized major f1 coat protein with infected host cell inner membrane.新合成的主要F1衣壳蛋白与受感染宿主细胞内膜的关联。
J Supramol Struct. 1972;1(1):8-18. doi: 10.1002/jss.400010103.
9
A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA.噬菌体M13病毒粒子的一种外壳蛋白参与DNA的膜定向合成。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):205-9. doi: 10.1073/pnas.70.1.205.
10
Reinvestigation of a region of the fd bacteriophage coat protein sequence.对fd噬菌体外壳蛋白序列的一个区域进行重新研究。
J Mol Biol. 1974 Sep 25;88(3):598-600. doi: 10.1016/0022-2836(74)90410-0.

M13噬菌体在病毒感染的大肠杆菌提取物中的膜相关组装。

Membrane-associated assembly of M13 phage in extracts of virus-infected Escherichia coli.

作者信息

Wickner W, Killick T

出版信息

Proc Natl Acad Sci U S A. 1977 Feb;74(2):505-9. doi: 10.1073/pnas.74.2.505.

DOI:10.1073/pnas.74.2.505
PMID:15248
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392318/
Abstract

Assembly of coliphage M13 is known to occur as the viral DNA crosses the cytoplasmic membrane, shedding its virus-coded DNA unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein. Conditions are described in which extracts of M13-infected E. coli and membranes prepared from such extracts will support virus assembly at a rate equivalent to that of intact cells. Extracts prepared from cells infected with temperature-sensitive M13 mutants in genes 1, 3, 4, or 5 are temperature-sensitive in this cell-free assembly reaction. Phage assembly in vitro requires magnesium and as yet an unidentified heat-stable cofactor of low molecular weight. The rate of virus assembly is approximately linear with respect to extract concentration over a 10(4)-fold range, consistent with the observation that the entire M13 assembly activity copurifies with the cell membrane fraction.

摘要

已知噬菌体M13的组装发生在病毒DNA穿过细胞质膜时，此时它会脱落病毒编码的DNA解旋蛋白，并从膜上获取大约2400份主要衣壳蛋白。文中描述了这样的条件：用M13感染的大肠杆菌提取物以及由此制备的膜，能以与完整细胞相当的速率支持病毒组装。用基因1、3、4或5中温度敏感型M13突变体感染细胞制备的提取物，在这种无细胞组装反应中对温度敏感。体外噬菌体组装需要镁以及一种尚未鉴定出的低分子量热稳定辅因子。在10^4倍的范围内，病毒组装速率与提取物浓度大致呈线性关系，这与整个M13组装活性与细胞膜部分共纯化的观察结果一致。