Suppr超能文献

体外phiX174依赖DNA的DNA合成：大肠杆菌dnaB突变提取物中P1禁令蛋白的需求

phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.

作者信息

Schuster H, Mikolajczyk M, Rohrschneider J, Geschke B

出版信息

Proc Natl Acad Sci U S A. 1975 Oct;72(10):3907-11. doi: 10.1073/pnas.72.10.3907.

DOI:10.1073/pnas.72.10.3907
PMID:670
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433105/
Abstract

Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is stimulated by KCl. Such soluble enzyme fractions were prepared from E. coli strains carrying the prophage mutant P1bac, in which the viral dnaB analog (ban) protein is expressed constitutively, or P1bacban, in which the expression of ban protein is prevented. DNA-synthesizing activity of ban protein containing fractions from wild-type or dnaB(P1bac) lysogens was more temperature-resistant than that from E. coli containing only wild-type dnaB protein, whereas that from dnaB(P1bacban) lysogens of dnaB cells was extremely thermolabile. It is suggested that the temperature-resistant DNA synthesis with fractions from P1bac lysogens is mediated by the P1 ban protein.

摘要

用硫酸铵对大肠杆菌粗提物进行分级分离，可得到一种可溶性酶级分（约25倍纯化），该酶级分催化φX174单链DNA转化为双链DNA。该反应对利福平具有抗性，需要单链DNA、Mg++、脱氧核苷三磷酸和ATP，并受KCl刺激。这种可溶性酶级分是从携带原噬菌体突变体P1bac的大肠杆菌菌株制备的，其中病毒dnaB类似物（ban）蛋白组成性表达，或从P1bacban制备的，其中ban蛋白的表达被阻止。来自野生型或dnaB（P1bac）溶原菌的含ban蛋白级分的DNA合成活性比仅含野生型dnaB蛋白的大肠杆菌的DNA合成活性更耐温度，而来自dnaB细胞的dnaB（P1bacban）溶原菌的DNA合成活性则极其不耐热。提示来自P1bac溶原菌级分的耐温度DNA合成是由P1 ban蛋白介导的。

相似文献

1
phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.体外phiX174依赖DNA的DNA合成：大肠杆菌dnaB突变提取物中P1禁令蛋白的需求
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3907-11. doi: 10.1073/pnas.72.10.3907.
2
Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli.在大肠杆菌提取物中将噬菌体φX174和fd单链DNA转化为复制形式。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233-7. doi: 10.1073/pnas.69.11.3233.
3
Bacteriophage phiX174 single-stranded viral DNA synthesis in temperature-sensitive dnaB and dna C mutants of Escherichia coli.噬菌体φX174单链病毒DNA在大肠杆菌温度敏感型dnaB和dnaC突变体中的合成
J Virol. 1976 May;18(2):426-35. doi: 10.1128/JVI.18.2.426-435.1976.
4
A dnaB analog ban, specified by bacteriophage P1: genetic and physiological evidence for functional analogy and interactions between the two products.噬菌体P1指定的dnaB类似物禁令：两种产物之间功能类似性及相互作用的遗传学和生理学证据
Mol Gen Genet. 1979 Jul 13;174(2):173-88. doi: 10.1007/BF00268354.
5
dnaG gene product, a rifampicin-resistant RNA polymerase, initiates the conversion of a single-stranded coliphage DNA to its duplex replicative form.dnaG基因产物，一种耐利福平的RNA聚合酶，启动单链大肠杆菌噬菌体DNA向其双链复制形式的转化。
J Biol Chem. 1975 Aug 10;250(15):5995-6001.
6
Synthesis of P1 ban protein in minicells infected by P1 mutants.P1突变体感染的微小细胞中P1禁令蛋白的合成。
Mol Gen Genet. 1980 Jan;177(2):193-7. doi: 10.1007/BF00267429.
7
DNA synthesis in an Escherichia coli dna B dnaC mutant.大肠杆菌dna B dnaC突变体中的DNA合成
Mol Gen Genet. 1977 Feb 28;151(1):11-6. doi: 10.1007/BF00446907.
8
The isolation and characterization of escherichia coli dnaB::Tn10 insertion mutations.大肠杆菌dnaB::Tn10插入突变的分离与鉴定
Mol Gen Genet. 1981;182(1):112-8. doi: 10.1007/BF00422776.
9
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
10
Involvement of host cell gene products in conversion of bacteriophage S13 single-stranded DNa to duplex replicative form DNA in vitro.宿主细胞基因产物在体外将噬菌体S13单链DNA转化为双链复制型DNA过程中的作用。
J Biochem. 1981 Sep;90(3):779-83. doi: 10.1093/oxfordjournals.jbchem.a133533.

引用本文的文献

1
Cloning, expression, and characterization of the icd gene in the immI operon of bacteriophage P1.噬菌体P1的immI操纵子中icd基因的克隆、表达及特性分析
J Bacteriol. 1993 May;175(10):2833-8. doi: 10.1128/jb.175.10.2833-2838.1993.
2
Phage P4 DNA replication in vitro.噬菌体P4 DNA的体外复制
Nucleic Acids Res. 1994 Jun 11;22(11):2065-70. doi: 10.1093/nar/22.11.2065.
3
Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.广宿主范围质粒RSF1010在大肠杆菌和铜绿假单胞菌无细胞提取物中的复制
Nucleic Acids Res. 1982 Aug 11;10(15):4687-702. doi: 10.1093/nar/10.15.4687.
4
Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins.广宿主范围质粒RSF1010的复制：对三种质粒编码蛋白的需求。
Proc Natl Acad Sci U S A. 1984 Feb;81(3):654-8. doi: 10.1073/pnas.81.3.654.
5
The dnaC protein of Escherichia coli. Purification, physical properties and interaction with dnaB protein.大肠杆菌的dnaC蛋白。纯化、物理性质及与dnaB蛋白的相互作用。
Nucleic Acids Res. 1983 Feb 25;11(4):987-97. doi: 10.1093/nar/11.4.987.
6
Discriminatory function of ribonuclease H in the selective initiation of plasmid DNA replication.核糖核酸酶H在质粒DNA复制选择性起始中的鉴别功能。
Nucleic Acids Res. 1982 Feb 11;10(3):833-53. doi: 10.1093/nar/10.3.833.
7
Rifampicin-resistant initiation of DNA synthesis on the isolated strands of ColE plasmid DNA.在ColE质粒DNA的单链上，利福平抗性的DNA合成起始。
Nucleic Acids Res. 1981 Oct 24;9(20):5215-31. doi: 10.1093/nar/9.20.5215.
8
Plasmid RSF1010 DNA replication in vitro promoted by purified RSF1010 RepA, RepB and RepC proteins.纯化的RSF1010 RepA、RepB和RepC蛋白促进体外质粒RSF1010 DNA复制。
Nucleic Acids Res. 1991 Mar 25;19(6):1203-11. doi: 10.1093/nar/19.6.1203.
9
Replication of small plasmids in extracts of Escherichia coli.小质粒在大肠杆菌提取物中的复制
Mol Gen Genet. 1976 Jun 15;145(3):273-80. doi: 10.1007/BF00325823.
10
A DNA primase specified by I-like plasmids.一种由I类质粒所指定的DNA引发酶。
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3632-6. doi: 10.1073/pnas.76.8.3632.

本文引用的文献

1
DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.肠道细菌的耐药性。IV. R因子与噬菌体P1结合产生的活性转导噬菌体P1 CM
J Bacteriol. 1964 Nov;88(5):1266-76. doi: 10.1128/jb.88.5.1266-1276.1964.
2
The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene.噬菌体φ-X174的感染过程。X. φ-X裂解基因中的突变
J Mol Biol. 1966 Jul;18(3):429-47. doi: 10.1016/s0022-2836(66)80035-9.
3
Escherichia coli mutants temperature-sensitive for DNA synthesis.对DNA合成温度敏感的大肠杆菌突变体。
Mol Gen Genet. 1971;113(3):273-84. doi: 10.1007/BF00339547.
4
The fate of newly made DNA in Escherichia coli mutants thermosensitive in DNA synthesis.大肠杆菌中DNA合成温度敏感型突变体中新合成DNA的命运
Mol Gen Genet. 1971;110(3):272-88. doi: 10.1007/BF00337839.
5
Replication of bacteriophages in Escherichia coli mutants thermosensitive in DNA synthesis.噬菌体在DNA合成中对温度敏感的大肠杆菌突变体中的复制。
Mol Gen Genet. 1970;106(3):274-85. doi: 10.1007/BF00340386.
6
Inhibition of synthesis of phiX174 DNA in a mutant host thermosensitive for DNA synthesis.在对DNA合成温度敏感的突变宿主中抑制φX174 DNA的合成。
J Mol Biol. 1968 Nov 14;37(3):525-8. doi: 10.1016/0022-2836(68)90120-4.
7
Multienzyme systems of DNA replication.DNA复制的多酶系统
Science. 1974 Dec 13;186(4168):987-93. doi: 10.1126/science.186.4168.987.
8
Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.用纯化的大肠杆菌蛋白将φX174病毒DNA转化为双链形式。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4. doi: 10.1073/pnas.71.10.4120.
9
Isolation of a complementing activity for a dna-B mutant.DNA-B突变体互补活性的分离
Biochem Biophys Res Commun. 1973 Aug 6;53(3):858-68. doi: 10.1016/0006-291x(73)90172-1.
10
Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.脱氧核糖核酸复制缺陷的温度敏感型大肠杆菌突变体的分离与鉴定。
J Bacteriol. 1973 Mar;113(3):1381-8. doi: 10.1128/jb.113.3.1381-1388.1973.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验