Jazwinski S M, Marco R, Kornberg A
Proc Natl Acad Sci U S A. 1973 Jan;70(1):205-9. doi: 10.1073/pnas.70.1.205.
Several molecules of a protein specified by gene 3 of M13 comprise a minor fraction of the phage coat and have been assigned a role in adsorption to the bacterial cell. We find that the gene-3 protein molecules of the virion are fully conserved in phage that have attached irreversibly to the host cell, and they form a complex with the phage DNA when it has been converted to a duplex replicative form. In cells infected at a restrictive temperature with a thermosensitive mutant in gene 3, there is no conversion of the phage DNA to the replicative form. Both the adsorbed phage and the complex of replicative form DNA with the gene-3 protein were isolated with the inner membrane fraction of the cell. We suggest that the gene-3 protein may perform an essential function in the synthesis of replicative form by linking the phage DNA to a cellular replicative system in or on the inner cell membrane.
由M13基因3所指定的一种蛋白质的几个分子构成噬菌体外壳的一小部分，并已被确定在吸附到细菌细胞中起作用。我们发现，病毒粒子的基因3蛋白质分子在不可逆地附着于宿主细胞的噬菌体中是完全保守的，并且当噬菌体DNA已转化为双链复制形式时，它们会与噬菌体DNA形成复合物。在用基因3中的温度敏感突变体在限制温度下感染的细胞中，噬菌体DNA不会转化为复制形式。吸附的噬菌体以及复制形式的DNA与基因3蛋白质的复合物都与细胞的内膜部分一起被分离出来。我们认为，基因3蛋白质可能通过将噬菌体DNA连接到细胞内膜内或其上的细胞复制系统，在复制形式的合成中发挥重要作用。
