Fuks Z, Vlodavsky I, Andreeff M, McLoughlin M, Haimovitz-Friedman A
Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
Eur J Cancer. 1992;28A(4-5):725-31. doi: 10.1016/0959-8049(92)90104-a.
The study demonstrates that the sensitivity of endothelial cells to irradiation in vitro is significantly affected by the microenvironmental conditions under which the experiment is carried out. When irradiated plateau phase bovine aortic endothelial cells were assayed for colony formation on top of uncoated plastic of standard culture dishes, the dose survival curves showed Do values of [mean (S.D.)] 107 (6) cGy and Dq of 63 (28) cGy (calculated according to the single-hit multitarget model). When assayed in dishes precoated with the autologous natural basement membrane-like extracellular matrix (BAEC/ECM) the curves showed a similar Do [106 (2) cGy], but the Dq was 194 (8) cGy (P less than 0.05), indicating that components of natural ECM confer in endothelial cells an improved capacity to repair radiation lesions and to restore the clonogenic capacity. However, when the natural but biologically unrelated HR9-bFGF/ECM was used, a decreased repair capacity was noted with Dq of 156 (30) cGy (P less than 0.05 compared with BAEC/ECM). The data demonstrate the high specificity of the repair function to interactions with autologous matrix components, and emphasise the need to select relevant experimental conditions when parameters of the radiation response in vitro are used to predict the response in vivo.
该研究表明，体外实验中内皮细胞对辐射的敏感性受实验所处微环境条件的显著影响。当检测处于平台期的牛主动脉内皮细胞在标准培养皿未包被塑料表面形成集落的情况时，剂量存活曲线显示，[均值（标准差）]Do值为107（6）cGy，Dq为63（28）cGy（根据单击多靶模型计算）。当在预先包被有自体天然基底膜样细胞外基质（BAEC/ECM）的培养皿中检测时，曲线显示出相似的Do[106（2）cGy]，但Dq为194（8）cGy（P小于0.05），这表明天然ECM的成分赋予内皮细胞更强的修复辐射损伤及恢复克隆形成能力。然而，当使用天然但生物学上不相关的HR9 - bFGF/ECM时，观察到修复能力下降，Dq为156（30）cGy（与BAEC/ECM相比，P小于0.05）。这些数据证明了修复功能与自体基质成分相互作用的高度特异性，并强调在使用体外辐射反应参数预测体内反应时选择相关实验条件的必要性。
