Sánchez L M, Vizoso F, Allende M T, Ruibal A, López-Otín C
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Eur J Cancer. 1992;28A(4-5):828-32. doi: 10.1016/0959-8049(92)90124-k.
Cyst fluids from 55 premenopausal women with gross cystic breast disease were classified by K+/Na+ ratio: 19 with ratio over 1 (type I) and 36 with ratio less than 1 (type II). Immunoradiometric assay of cathepsin D in both types of cyst fluids revealed the presence of large amounts of this proteinase. The average concentration of cathepsin D in type I cyst fluids was 63.3 nmol/l, which was significantly higher than that corresponding to type II cyst fluids (35.1 nmol/l). Immunoprecipitation analysis of intracystic cathepsin D demonstrated that this protein was present as the 52 kD non-processed precursor form of the molecule. Since procathepsin D is a useful prognostic marker in breast carcinoma, we suggest that cyst fluid quantification of cathepsin D could aid to detect patients affecting of gross cystic disease with higher risk for developing breast cancer.
对55名患有乳腺大囊性病的绝经前女性的囊液，根据钾离子/钠离子比值进行分类：19名女性的比值大于1（I型），36名女性的比值小于1（II型）。对两种类型囊液中的组织蛋白酶D进行免疫放射分析，结果显示存在大量这种蛋白酶。I型囊液中组织蛋白酶D的平均浓度为63.3纳摩尔/升，显著高于II型囊液相应的浓度（35.1纳摩尔/升）。对囊内组织蛋白酶D的免疫沉淀分析表明，该蛋白以分子的52kD未加工前体形式存在。由于组织蛋白酶D原是乳腺癌中一种有用的预后标志物，我们认为对囊液中组织蛋白酶D进行定量分析有助于检测出患有乳腺大囊性病且患乳腺癌风险较高的患者。
