Diamandis E P, Yu H, López-Otín C
Department of Pathology, Mount Sinai Hospital, Toronto, Ontario, Canada.
Breast Cancer Res Treat. 1996;38(3):259-64. doi: 10.1007/BF01806144.
We demonstrate for the first time that prostate specific antigen (PSA) is a component of breast cyst fluid. At the cutoff level of 0.01 or 0.03 micrograms/L of PSA, 64% or 43% of cyst fluids are positive for PSA, respectively. PSA in cyst fluid, as characterized by high performance liquid chromatography, exists in almost equal concentrations as free PSA, with a molecular weight of 33 KDa, and as PSA bound to alpha 1-antichymotrypsin, with a molecular weight of 100 KDa. PSA presence was also characterized in cyst fluid by Western blot analysis. These data suggest that PSA is a frequent component of breast cyst fluid. More studies are needed to establish the role of this serine protease in normal breast, gross breast cystic disease, and breast cancer.
我们首次证明前列腺特异性抗原（PSA）是乳腺囊肿液的一个组成部分。在PSA浓度截止值为0.01或0.03微克/升时，分别有64%或43%的囊肿液PSA呈阳性。通过高效液相色谱法分析，囊肿液中的PSA以几乎相等的浓度存在，一种是分子量为33千道尔顿的游离PSA，另一种是分子量为100千道尔顿的与α1 -抗糜蛋白酶结合的PSA。通过蛋白质印迹分析也证实了囊肿液中存在PSA。这些数据表明PSA是乳腺囊肿液中常见的成分。需要更多研究来确定这种丝氨酸蛋白酶在正常乳腺、乳腺大囊性疾病和乳腺癌中的作用。
