1,6-二磷酸果糖在大鼠肝移植肝脏保存冷保存液中的保护作用
Protective effect of fructose-1,6-bisphosphate in the cold storage solution for liver preservation in rat hepatic transplantation.
作者信息
Moresco R N, Santos R C V, Alves Filho J C F, Cunha A A, Dos Reis C, Reichel C L, De Oliveira J R
机构信息
Laboratório de Pesquisa em Biofísica, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Transplant Proc. 2004 Jun;36(5):1261-4. doi: 10.1016/j.transproceed.2004.05.040.
Fructose-1,6-bisphosphate (FBP) has been reported to have a protective effect on liver injury following ischemic/reperfusion periods. FBP maintains ATP levels and thereby cellular energy metabolism, which is important to the liver during cold preservation. In the present study, we evaluated the effects of FBP on the composition of storage solutions for cold liver preservation. Adult male Wistar rats were randomly divided into three experimental groups. Hepatic perfusion and preservation were performed with UW, UW plus 10 mmol/L FBP (UWM), and FBP 10 mmol/L (FBPS) alone solutions. Biochemical measurements of AST, ALT, and TBARS were performed on samples of the cold storage solution at 0, 12, 18, and 24 hours preservation. FBPS and UW solutions showed similar preservation grades during 18 hours. Addition of 10 mmol/L of FBP to UW solution induced liver injury and a poor preservation grade. FBP appears to protect the liver from injury caused by free radicals when the preservation time is less than 18 hours. Therefore, FBP may exert a protective effect for the preservation of livers during cold storage, and could represent an important component of new cold storage solutions.
据报道，1,6-二磷酸果糖（FBP）对缺血/再灌注后的肝损伤具有保护作用。FBP维持ATP水平，从而维持细胞能量代谢，这在肝脏冷保存期间对肝脏很重要。在本研究中，我们评估了FBP对冷肝保存液成分的影响。成年雄性Wistar大鼠被随机分为三个实验组。分别用UW液、UW加10 mmol/L FBP的溶液（UWM）和单独的10 mmol/L FBP溶液（FBPS）进行肝脏灌注和保存。在保存0、12、18和24小时时，对冷保存液样本进行AST、ALT和TBARS的生化检测。在18小时内，FBPS溶液和UW溶液显示出相似的保存等级。向UW溶液中添加10 mmol/L的FBP会导致肝损伤和较差的保存等级。当保存时间小于18小时时，FBP似乎可以保护肝脏免受自由基引起的损伤。因此，FBP可能对肝脏冷保存起到保护作用，并且可能成为新型冷保存液的重要成分。
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