Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Avenida Roraima 1000, Prédio 26, Sala 1401, Camobi, 97105-900 Santa Maria, Rio Grande do Sul, Brazil.
Inflammation. 2012 Dec;35(6):1786-92. doi: 10.1007/s10753-012-9498-6.
The accumulation of advanced oxidation protein products (AOPP) has been linked to several pathological conditions. Previous studies have identified AOPP as a novel biomarker of oxidative damage to proteins and a novel class of mediator of inflammation. The aim of this study was to determine the effects of fructose-1,6-bisphosphate (FBP) and N-acetylcysteine (NAC) as well as the synergistic effect of both treatments on the formation of AOPP in vitro. For this purpose, we incubated the human serum albumin (HSA) with various hypochlorous acid (HOCl) concentrations to produce albumin-advanced oxidation protein products (HSA-AOPP). Both FBP and NAC were capable of inhibiting the formation of HOCl-induced AOPP in a concentration-dependent manner. The synergistic effect promoted by the association of these drugs showed to be more effective than when tested alone. Thus, both FBP and NAC may be good candidates to mitigate and neutralize pro-inflammatory and pro-oxidant effects of AOPP in several diseases.
先进氧化蛋白产物 (AOPP) 的积累与多种病理状况有关。先前的研究已经确定 AOPP 是蛋白质氧化损伤的新型生物标志物和炎症的新型介质。本研究旨在确定 1,6-二磷酸果糖 (FBP) 和 N-乙酰半胱氨酸 (NAC) 以及两种治疗方法的协同作用对体外 AOPP 形成的影响。为此，我们用各种次氯酸 (HOCl) 浓度孵育人血清白蛋白 (HSA) 以产生白蛋白-先进氧化蛋白产物 (HSA-AOPP)。FBP 和 NAC 都能够以浓度依赖的方式抑制 HOCl 诱导的 AOPP 的形成。这些药物联合使用所产生的协同作用比单独测试时更有效。因此，FBP 和 NAC 都可能是减轻和中和几种疾病中 AOPP 的促炎和促氧化作用的良好候选药物。
