恶性黑色素瘤组织芯片和完整切片中DNA倍体状态及DNA倍体相关参数的比较

Comparison of DNA ploidy status and DNA ploidy-related parameters in malignant melanoma tissue microarrays and full sections.

作者信息

Korabiowska Monika, Cordon-Cardo Carlos, Buschmann Nadine, Stachura Jerzy, Fischer Gösta, Brinck Ulrich

机构信息

Department of Cytopathology, University of Göttingen, Göttingen, Germany.

出版信息

Hum Pathol. 2004 Jul;35(7):887-91. doi: 10.1016/j.humpath.2004.03.015.

DOI:10.1016/j.humpath.2004.03.015

PMID:15257554

Abstract

A new high-throughput tissue-arraying technique, now frequently used in tumor pathology, requires standardization of methods of DNA analysis, previously applied in full histological sections. The main objectives of this study were to evaluate DNA ploidy status and DNA ploidy-related parameters using the CAS200 image analyzer in malignant melanoma tissue microarrays and to compare them with full histological sections. Comparison of DNA ploidy-related parameters, including percentage of diploid cells, percentage of aneuploid cells between 2c and 4c, percentage of tetraploid cells, percentage of aneuploid cells between 4c and 8c, percentage of octaploid cells, percentage of 16-ploid cells, and 5c exceeding rate, did not reveal any significant differences between malignant melanoma tissue microarrays and full sections. The DNA ploidy status according to Auer differed in 1 out of 59 cases investigated. Our study demonstrated that it is possible to evaluate DNA ploidy status and DNA ploidy-related parameters in tissue microarrays, which is of practical relevance to tumor pathology.

摘要

一种新的高通量组织阵列技术目前在肿瘤病理学中经常使用，这就需要对以前应用于完整组织学切片的DNA分析方法进行标准化。本研究的主要目的是使用CAS200图像分析仪评估恶性黑色素瘤组织微阵列中的DNA倍体状态和与DNA倍体相关的参数，并将它们与完整组织学切片进行比较。对与DNA倍体相关的参数进行比较，包括二倍体细胞百分比、2c至4c之间的非整倍体细胞百分比、四倍体细胞百分比、4c至8c之间的非整倍体细胞百分比、八倍体细胞百分比、16倍体细胞百分比和5c超标率，结果显示恶性黑色素瘤组织微阵列和完整切片之间没有任何显著差异。在所调查的59例病例中，有1例根据奥尔（Auer）分类的DNA倍体状态有所不同。我们的研究表明，在组织微阵列中评估DNA倍体状态和与DNA倍体相关的参数是可行的，这对肿瘤病理学具有实际意义。