Medina Araceli, Swain Rajeeb K, Kuerner Klaus-Michael, Steinbeisser Herbert
Department of Cell Biology, Max-Planck-Institute for Developmental Biology, Tübingen, Germany.
EMBO J. 2004 Aug 18;23(16):3249-58. doi: 10.1038/sj.emboj.7600329. Epub 2004 Jul 22.
Protocadherins have homophilic adhesion properties and mediate selective cell-cell adhesion and cell sorting. Knockdown of paraxial protocadherin (PAPC) function in the Xenopus embryo impairs tissue separation, a process that regulates separation of cells of ectodermal and mesodermal origin during gastrulation. We show that PAPC can modulate the activity of the Rho GTPase and c-jun N-terminal kinase, two regulators of the cytoskeletal architecture and effectors of the planar cell polarity pathway. This novel signaling function of PAPC is essential for the regulation of tissue separation. In addition, PAPC can interact with the Xenopus Frizzled 7 receptor, and both proteins contribute to the development of separation behavior by activating Rho and protein kinase Calpha.
原钙黏蛋白具有嗜同性黏附特性,介导选择性细胞间黏附和细胞分选。在非洲爪蟾胚胎中敲低近轴原钙黏蛋白(PAPC)的功能会损害组织分离,这一过程在原肠胚形成期间调节外胚层和中胚层来源细胞的分离。我们发现,PAPC可以调节Rho GTP酶和c-jun N端激酶的活性,这两种酶是细胞骨架结构的调节因子以及平面细胞极性通路的效应器。PAPC的这种新的信号传导功能对于组织分离的调节至关重要。此外,PAPC可以与非洲爪蟾卷曲蛋白7受体相互作用,并且这两种蛋白质都通过激活Rho和蛋白激酶Cα来促进分离行为的发展。