Wang Yingqun, Janicki Patricia, Köster Isabelle, Berger Corinna D, Wenzl Christian, Grosshans Jörg, Steinbeisser Herbert
Institute of Human Genetics, University Heidelberg, 69120 Heidelberg, Germany.
Genes Dev. 2008 Apr 1;22(7):878-83. doi: 10.1101/gad.452908.
Xenopus Paraxial Protocadherin (xPAPC) has signaling functions that are essential for convergent extension (CE) movements and tissue separation during gastrulation. PAPC modulates components of the planar cell polarity (PCP) pathway, but it is not clear how PAPC is connected to beta-catenin-independent Wnt-signaling. By yeast two-hybrid screen, we found that the intracellular domain of PAPC interacts with Sprouty (Spry), an inhibitor of CE movements. Upon binding to PAPC, Spry function is inhibited and PCP signaling is enhanced. Our data indicate that PAPC promotes gastrulation movements by sequestration of Spry and reveal a novel mechanism by which protocadherins modulate beta-catenin-independent Wnt-signaling.
非洲爪蟾原轴突原钙黏蛋白(xPAPC)具有信号传导功能,这些功能对于原肠胚形成期间的汇聚延伸(CE)运动和组织分离至关重要。PAPC调节平面细胞极性(PCP)途径的组成部分,但尚不清楚PAPC如何与非β-连环蛋白依赖性Wnt信号传导相联系。通过酵母双杂交筛选,我们发现PAPC的细胞内结构域与CE运动的抑制剂Sprouty(Spry)相互作用。与PAPC结合后,Spry功能受到抑制,PCP信号传导增强。我们的数据表明,PAPC通过隔离Spry促进原肠胚形成运动,并揭示了原钙黏蛋白调节非β-连环蛋白依赖性Wnt信号传导的新机制。