在向碳水化合物耗尽的酿酒酵母重新添加葡萄糖后，胞质钙的细胞内耗散。

The intracellular dissipation of cytosolic calcium following glucose re-addition to carbohydrate depleted Saccharomyces cerevisiae.

作者信息

Kellermayer Richard, Szigeti Réka, Kellermayer Miklós, Miseta Attila

机构信息

Department of Medical Genetics and Child Development Medicine, University of Pécs, Szigeti ut 12, 7623 Pécs, Hungary.

出版信息

FEBS Lett. 2004 Jul 30;571(1-3):55-60. doi: 10.1016/j.febslet.2004.06.049.

DOI:10.1016/j.febslet.2004.06.049

PMID:15280017

Abstract

Glucose re-addition to carbohydrate starved yeast cells leads to a transient elevation of eytosolic calcium (TECC). Concomitantly, a cytosolic proton extrusion occurs through the activation of the vacuolar H(+)-ATPase and the plasma membrane H(+)-ATPases. This study addressed the dissipation of the TECC through intracellular compartmentalization and the possible affects of the H(+)-ATPases on this process. Both the vacuole and the Golgi-ER apparatus were found to play important roles in distributing calcium to internal stores. Additionally, the inhibition of cytosolic proton extrusion augmented cytosolic calcium responses. A model where pH dependent cytosolic calcium buffering plays an important role in the dissipation of the TECC in Saccharomyces cerevisiae is proposed.

摘要

向碳水化合物饥饿的酵母细胞重新添加葡萄糖会导致胞质钙短暂升高（TECC）。与此同时，通过液泡H(+)-ATP酶和质膜H(+)-ATP酶的激活会发生胞质质子外排。本研究探讨了通过细胞内区室化实现的TECC消散以及H(+)-ATP酶对该过程可能产生的影响。发现液泡和高尔基体-内质网装置在将钙分配到内部储存中都起着重要作用。此外，抑制胞质质子外排会增强胞质钙反应。提出了一个模型，其中pH依赖性胞质钙缓冲在酿酒酵母中TECC的消散中起重要作用。