Feurer Carole, Clermont Dominique, Bimet François, Candréa Adina, Jackson Mary, Glaser Philippe, Bizet Chantal, Dauga Catherine
Laboratoire de Génomique des Micro-organismes Pathogènes, Département Structure et Dynamique des Génomes, Institut Pasteur, 25-28 rue du Dr Roux, 75724 Paris cedex 15, France.
Molecular Diagnostics Unit, Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Cork, Ireland.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1055-1061. doi: 10.1099/ijs.0.02907-0.
Nine unidentified Gram-positive, lipophilic corynebacteria were isolated from clinical and food samples and subjected to a polyphasic taxonomic analysis. The bacteria were distinguished from Corynebacterium species with validly published names by biochemical tests, fatty acid content and whole-cell protein analysis. Comparative 16S rRNA gene sequence analysis demonstrated unambiguously that the nine strains were related phylogenetically to the species 'Corynebacterium tuberculostearicum' and represented a distinct subline within the genus Corynebacterium. On the basis of both phenotypic and phylogenetic evidence, the formal description of Corynebacterium tuberculostearicum sp. nov. is proposed. The type strain of C. tuberculostearicum is Medalle XT (=LDC-20T=CIP 107291T=CCUG 45418T=ATCC 35529T).
从临床和食品样本中分离出9株未鉴定的革兰氏阳性嗜脂性棒状杆菌，并进行了多相分类分析。通过生化试验、脂肪酸含量和全细胞蛋白分析，将这些细菌与已有效发表名称的棒状杆菌属菌种区分开来。比较16S rRNA基因序列分析明确表明，这9株菌株在系统发育上与“结核硬脂酸棒状杆菌”相关，代表了棒状杆菌属内一个独特的亚系。基于表型和系统发育证据，提出了结核硬脂酸棒状杆菌新种的正式描述。结核硬脂酸棒状杆菌的模式菌株是Medalle XT（=LDC-20T=CIP 107291T=CCUG 45418T=ATCC 35529T）。
