Fernández-Garayzábal J F, Vela A I, Egido R, Hutson R A, Lanzarot M P, Fernández-García M, Collins M D
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
School of Food Biosciences, University of Reading, Reading RG6 6AP, UK.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2191-2195. doi: 10.1099/ijs.0.63165-0.
Eight unidentified Gram-positive, rod-shaped organisms were recovered from the tracheas of apparently healthy black storks (Ciconia nigra) and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria the isolates were tentatively assigned to the genus Corynebacterium, although three of the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus Corynebacterium. Five strains were genotypically identified as representing Corynebacterium falsenii, whereas the remaining three strains represented a hitherto unknown subline, associated with a small subcluster of species that includes Corynebacterium mastitidis and its close relatives. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from black storks represent a novel species within the genus Corynebacterium, for which the Corynebacterium ciconiae sp. nov. is proposed. The type strain is CECT 5779(T) (=BS13(T)=CCUG 47525(T)).
从看似健康的黑鹳（黑鹳属）气管中分离出8株未鉴定的革兰氏阳性杆状菌，并对其进行了多相分类分析。根据细胞形态和生化标准，这些分离株初步被归类为棒状杆菌属，尽管其中3株菌似乎与任何已知物种都不相符。比较16S rRNA基因测序研究表明，所有分离株在系统发育上均属于棒状杆菌属。5株菌株经基因分型鉴定为假棒状杆菌，其余3株代表一个迄今未知的亚系，与包括乳腺炎棒状杆菌及其近缘种在内的一小类物种相关。基于表型和系统发育证据，建议将从黑鹳中分离出的未知菌株归为棒状杆菌属内的一个新物种，命名为黑鹳棒状杆菌（Corynebacterium ciconiae sp. nov.）。模式菌株为CECT 5779(T)（=BS13(T)=CCUG 47525(T)）。
