Igarashi E, Kawamura N, Takeshita S
Developmental Laboratories, Marion Merrell Dow K.K., Osaka, Japan.
Biotech Histochem. 1994 Nov;69(6):305-10. doi: 10.3109/10520299409106310.
A method for observing whole rat fetal viscera embedded in gelatin using an automatic slicing apparatus is described. Fetuses were immersed in Bouin's solution. Part of the thoracic and abdominal skin of each fetus was removed, and fetuses were immersed consecutively in sodium bicarbonate 30% in 70% ethanol, gelatin 15% in water, gelatin 30% in water, then embedded in fresh 30% gelatin. The gelatin blocks containing the fetuses were immersed in 10% formalin. After fixation, the block was sliced into 200 microns serial transverse sections using a rotor-slicer at a rotation speed of 120 rpm and a cutting speed of 25 mm/sec. Complete slicing of a single fetus required about 20 min. The advantages of the method presented here include: complete fetal serial sections are produced, thin and uniform sections are obtained easily, viscera can be identified easily, and observation can be carried out at any time after slicing. The method presented can be used to detect whole fetal visceral malformations in developmental toxicity tests.
描述了一种使用自动切片装置观察包埋在明胶中的大鼠全胎内脏的方法。胎儿浸入Bouin氏液中。去除每个胎儿的部分胸腹部皮肤，然后将胎儿依次浸入70%乙醇中的30%碳酸氢钠溶液、水中的15%明胶溶液、水中的30%明胶溶液，然后包埋在新鲜的30%明胶中。将含有胎儿的明胶块浸入10%福尔马林中。固定后，使用转子切片机以120转/分钟的转速和25毫米/秒的切割速度将明胶块切成200微米的连续横切片。完整切片一个胎儿大约需要20分钟。此处介绍的方法的优点包括：可制作完整的胎儿连续切片，易于获得薄而均匀的切片，易于识别内脏，并且切片后可随时进行观察。所介绍的方法可用于发育毒性试验中检测全胎内脏畸形。
