Vournakis John N, Demcheva Marina, Whitson Anne, Guirca Radu, Pariser Ernst R
Marine Polymer Technologies, Inc., Burlington, Massachusetts 01803, USA.
J Trauma. 2004 Jul;57(1 Suppl):S2-6. doi: 10.1097/01.ta.0000136741.66698.9d.
A new polymeric material, poly-N-acetyl glucosamine (p-GlcNAc) fiber, has been identified and is effective in achieving hemostasis in surgical procedures and trauma. The p-GlcNAc material is purified from large-scale cultures of a marine microalga.
Poly-N-acetyl glucosamine materials have been formulated as films, sponges, gels, and microspheres. The polymer's structure has been characterized by chemical composition, carbohydrate analysis, spectroscopic techniques, intrinsic viscosity, and electron microscopy.
Carbohydrate analyses indicate that the primary sugar present in p-GlcNAc is N-acetyl glucosamine. Elemental analyses yield percentage values for carbon, nitrogen, and hydrogen that support that the polymer is fully acetylated. Molecular weight determinations indicate that the polymer has a molecular weight of 2.0 x 10(6) Da. Fourier transform infrared, nuclear magnetic resonance, and circular dichroism spectral data have defined a unique tertiary structure. Biologic testing demonstrated that p-GlcNAc materials are fully biocompatible.
The p-GlcNAc fiber has a unique beta-tertiary structure.
一种新型聚合材料——聚-N-乙酰葡糖胺（p-GlcNAc）纤维已被鉴定出来，它在外科手术和创伤止血方面效果显著。p-GlcNAc材料是从一种海洋微藻的大规模培养物中纯化得到的。
聚-N-乙酰葡糖胺材料已被制成薄膜、海绵、凝胶和微球。该聚合物的结构已通过化学成分分析、碳水化合物分析、光谱技术、特性粘度和电子显微镜进行了表征。
碳水化合物分析表明，p-GlcNAc中存在的主要糖类是N-乙酰葡糖胺。元素分析得出的碳、氮和氢的百分比值支持该聚合物已完全乙酰化。分子量测定表明该聚合物的分子量为2.0×10⁶道尔顿。傅里叶变换红外光谱、核磁共振光谱和圆二色光谱数据确定了一种独特的三级结构。生物学测试表明p-GlcNAc材料具有完全的生物相容性。
p-GlcNAc纤维具有独特的β-三级结构。
