Thatte Hemant S, Zagarins Sofija E, Amiji Mansoor, Khuri Shukri F
Department Surgery, VA Boston Healthcare System, Brigham and Women's Hospital, Harvard Medical Schools, Boston, MA, USA.
J Trauma. 2004 Jul;57(1 Suppl):S7-12. doi: 10.1097/01.ta.0000136742.04816.38.
Investigations were performed to assess the effect of poly-N-acetyl glucosamine (p-GlcNAc) fiber slurry-mediated hemostasis by interactions with red blood cells.
Red blood cell aggregation studies were performed using test material-coated microscope slides and multiphoton microscopic measurements. Enzymatic removal of red blood cell surface proteins was achieved using trypsin and neuraminidase treatment. Zeta-potential measurements (surface charge) were performed.
Red blood cells interact directly with poly-N-acetyl glucosamine polymers through ionic interactions and cell-surface proteins. The effective concentration of poly-N-acetyl glucosamine fiber material for 50% red blood cell aggregation was 0.28 mg/mL. The p-GlcNAc beta-configuration fibers and an alpha-configuration structural modification of the fibers both produced maximal responses because of their zeta-potentials, whereas other chemically modified p-GlcNAcs and chitosans were ineffective.
Poly-N-acetyl glucosamine-induced red blood cell aggregation is mediated by interactions with red blood cell surface charges.
开展研究以评估聚-N-乙酰葡糖胺（p-GlcNAc）纤维浆液通过与红细胞相互作用实现止血的效果。
使用涂覆有测试材料的显微镜载玻片和多光子显微镜测量进行红细胞聚集研究。通过胰蛋白酶和神经氨酸酶处理实现红细胞表面蛋白的酶促去除。进行了zeta电位测量（表面电荷）。
红细胞通过离子相互作用和细胞表面蛋白直接与聚-N-乙酰葡糖胺聚合物相互作用。使50%红细胞聚集的聚-N-乙酰葡糖胺纤维材料的有效浓度为0.28 mg/mL。p-GlcNAc的β构型纤维和该纤维的α构型结构修饰因其zeta电位均产生最大反应，而其他化学修饰的p-GlcNAc和壳聚糖则无效。
聚-N-乙酰葡糖胺诱导的红细胞聚集是由与红细胞表面电荷的相互作用介导的。
