聚-N-乙酰葡糖胺对含和不含红细胞的富血小板血浆中血小板活化的体外作用。
In vitro effects of poly-N-acetyl glucosamine on the activation of platelets in platelet-rich plasma with and without red blood cells.
作者信息
Valeri C Robert, Srey Rithy, Tilahun Daniel, Ragno Gina
机构信息
Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
出版信息
J Trauma. 2004 Jul;57(1 Suppl):S22-5; discussion S25. doi: 10.1097/01.ta.0000136744.12440.47.
BACKGROUND
This study was performed to assess the effect of poly-N-acetyl glucosamine fiber slurry on plasma clotting proteins, platelets, and red blood cells in the clotting of the blood.
METHODS
Citrate phosphate dextrose whole blood was stored at 22degreesC for 48 hours to prepare platelet-poor plasma, platelet-rich plasma (PRP), and PRP plus red blood cells with hematocrit values of 20%, 35%, and 45% with and without an equal volume of poly-N-acetyl glucosamine fibers (1 mg/mL 0.9% NaCl).
RESULTS
Thromboelastogram data show that poly-N-acetyl glucosamine fibers (p-GlcNAc) significantly reduced the R time in platelet-poor plasma, PRP, and PRP supplemented with red blood cells. Poly-N-acetyl glucosamine fibers increased, but not significantly, Annexin V and factor X binding to platelets, platelet microparticles, and red blood cell Annexin V binding. Poly-N-acetyl glucosamine fibers increased the production of thromboxane B2 by PRP.
CONCLUSION
Poly-N-acetyl glucosamine slurry activates platelets.
背景
本研究旨在评估聚-N-乙酰葡糖胺纤维浆液对血液凝固过程中血浆凝血蛋白、血小板和红细胞的影响。
方法
将枸橼酸盐磷酸盐葡萄糖全血在22℃下储存48小时，以制备乏血小板血浆、富血小板血浆（PRP）以及血细胞比容值分别为20%、35%和45%的添加和不添加等体积聚-N-乙酰葡糖胺纤维（1mg/mL 0.9%氯化钠）的PRP加红细胞。
结果
血栓弹力图数据显示，聚-N-乙酰葡糖胺纤维（p-GlcNAc）显著缩短了乏血小板血浆、PRP以及添加红细胞的PRP中的R时间。聚-N-乙酰葡糖胺纤维增加了膜联蛋白V和因子X与血小板、血小板微粒的结合以及红细胞膜联蛋白V的结合，但增加幅度不显著。聚-N-乙酰葡糖胺纤维增加了PRP中血栓素B2的生成。
结论
聚-N-乙酰葡糖胺浆液可激活血小板。
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