Favuzza Joanne, Hechtman Herbert B
Department Surgery, Harvard Medical School, and Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
J Trauma. 2004 Jul;57(1 Suppl):S42-4. doi: 10.1097/01.ta.0000136745.89568.d9.
This study tests whether the hemostatic action of poly-N-acetyl glucosamine (p-GlcNAc) fiber material involves vasoconstrictor release leading to closure of an aortic laceration.
A 22-gauge cannula was inserted into an infrarenal aortic segment of a rat. Surrounding ligatures were tied, and the aorta was flushed with 60 mL of saline from a reservoir held at 80 cm. A 23-gauge aortic puncture was made. The time taken to empty the reservoir was recorded.
Control patches led to an emptying time of 295 seconds, whereas p-GlcNAc patches increased this time to greater than 600 seconds. Ten minutes after patch removal, the emptying time decreased to 330 seconds. The rats were treated intravenously with endothelin receptor antagonists BQ-485 or JKC-301. The emptying time shortened to control values, despite the use of the p-GlcNAc fiber-based patch.
The mechanism of hemostasis by poly-N-acetyl glucosamine involves endothelin release independent of formed elements of blood.
本研究旨在测试聚-N-乙酰葡糖胺（p-GlcNAc）纤维材料的止血作用是否涉及血管收缩剂释放，从而导致主动脉撕裂口闭合。
将一根22号套管插入大鼠肾下腹主动脉段。扎紧周围的结扎线，并用来自置于80厘米高处储液器中的60毫升生理盐水冲洗主动脉。进行23号主动脉穿刺。记录排空储液器所需的时间。
对照贴片导致排空时间为295秒，而p-GlcNAc贴片将此时间延长至超过600秒。移除贴片10分钟后，排空时间降至330秒。给大鼠静脉注射内皮素受体拮抗剂BQ-485或JKC-301。尽管使用了基于p-GlcNAc纤维的贴片，排空时间仍缩短至对照值。
聚-N-乙酰葡糖胺的止血机制涉及内皮素释放，与血液中的有形成分无关。
